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验证数据展示

  • IF Staining of HeLa using CL594-17441

    IF Staining of HeLa using CL594-17441

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 594 GALNT2 antibody (CL594-17441) at dilution of 1:200.

CoraLite® Plus 594-conjugated GALNT2 Polyclonal antibody

GALNT2 Polyclonal Antibody for IF/ICC
Cat No. CL594-17441

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

GalNAc T2, GALNT2, pp GaNTase 2

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GALNT2 Ready-To-Use IHC Kit. GALNT2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17441-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-17441 targets GALNT2 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag11523

Product name: Recombinant human GALNT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 222-571 aa of BC041120

Sequence: FLDSHCECNEHWLEPLLERVAEDRTRVVSPIIDVINMDNFQYVGASADLKGGFDWNLVFKWDYMTPEQRRSRQGNPVAPIKTPMIAGGLFVMDKFYFEELGKYDMMMDVWGGENLEISFRVWQCGGSLEIIPCSRVGHVFRKQHPYTFPGGSGTVFARNTRRAAEVWMDEYKNFYYAAVPSARNVPYGNIQSRLELRKKLSCKPFKWYLENVYPELRVPDHQDIAFGALQQGTNCLDTLGHFADGVVGVYECHNAGGNQEWALTKEKSVKHMDLCLTVVDRAPGSLIKLQGCRENDSRQKWEQIEGNSKLRHVGSNLCLDSRTAKSGGLSVEVCGPALSQQWKFTLNLQQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2)
别名 GalNAc T2, GALNT2, pp GaNTase 2
计算分子量 571 aa, 65 kDa
观测分子量64 kDa
GenBank蛋白编号BC041120
基因名称 GALNT2
Gene ID (NCBI) 2590
RRIDAB_3673427
偶联类型 CoraLite® Plus 594 Fluorescent Dye
最大激发/发射波长594 nm / 615 nm
形式Liquid
纯化方式Antigen Affinity Purified
UNIPROT IDQ10471
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GALNT2, a GalNAc-transferase involved in O-linked glycosylation, modulates the risk of atherogenic dyslipidemia, a specific hallmark of insulin resistance and acts as a positive modulator of insulin signaling in human liver cells by down-regulating ENPP1. GALNT2 gene encodes GalNAc-T2 as a regulator of high-density lipoprotein cholesterol (HDL-C) metabolism (PMID: 31040393, 27508872). Western blot analysis detected GALNT2 at an apparent molecular mass of 64 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 594 GALNT2 antibody CL594-17441Download protocol
Standard Protocols
Click here to view our Standard Protocols
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