验证数据展示
经过测试的应用
Positive WB detected in | A375 cells, HeLa cells, MCF-7 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 4 publications below |
IHC | See 2 publications below |
产品信息
14414-1-AP targets GALE in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse |
文献引用反应性 | human, rat |
免疫原 | GALE fusion protein Ag5756 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | UDP-galactose-4-epimerase |
别名 | UDP-GlcNAc 4-epimerase, UDP-GalNAc 4-epimerase, UDP-galactose 4-epimerase, SDR1E1, EC:5.1.3.7 |
计算分子量 | 38 kDa |
观测分子量 | 38 kDa |
GenBank蛋白编号 | BC050685 |
基因名称 | GALE |
Gene ID (NCBI) | 2582 |
RRID | AB_2108540 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q14376 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GALE(Galactowaldenase) is also named as UDP-glucose 4-epimerase, UDP-galactose 4-epimerase, and belongs to the sugar epimerase family. UDP-galactose 4-epimerase catalyzes the interconversion of UDP-glucose and UDP-galactose during normal galactose metabolism. In humans, deficiencies in this enzyme lead to the complex disorder referred to as epimerase-deficiency galactosemia(PMID:10801319).It can exsit as a homodimer(PMID:8931134).
实验方案
Product Specific Protocols | |
---|---|
WB protocol for GALE antibody 14414-1-AP | Download protocol |
IHC protocol for GALE antibody 14414-1-AP | Download protocol |
IF protocol for GALE antibody 14414-1-AP | Download protocol |
IP protocol for GALE antibody 14414-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Redox Biol Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation. | ||
PLoS One Angelica Sinensis Polysaccharides Stimulated UDP-Sugar Synthase Genes through Promoting Gene Expression of IGF-1 and IGF1R in Chondrocytes: Promoting Anti-Osteoarthritic Activity. | ||
Infect Drug Resist iTRAQ-Based Proteomics Reveals Potential Anti-Virulence Targets for ESBL-Producing Klebsiella pneumoniae. | ||
Biochem Biophys Res Commun The critical role of UDP-galactose-4-epimerase in osteoarthritis: modulating proteoglycans synthesis of articular chondrocytes.
|