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验证数据展示

  • WB analysis using 12945-1-AP (same clone as 12945-1-PBS)

    WB analysis using 12945-1-AP (same clone as 12945-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 12945-1-AP (GAGE7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12945-1-PBS in a different storage buffer formulation.

  • WB analysis of LNCaP using 12945-1-AP (same clone as 12945-1-PBS)

    WB analysis of LNCaP using 12945-1-AP (same clone as 12945-1-PBS)

    WB result of GAGE7 antibody (12945-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-GAGE7 transfected LNCaP cells. This data was developed using the same antibody clone with 12945-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 12945-1-AP (same clone as 12945-1-PBS)

    IHC staining of human gliomas using 12945-1-AP (same clone as 12945-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12945-1-AP (GAGE7 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12945-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 12945-1-AP (same clone as 12945-1-PBS)

    IHC staining of human gliomas using 12945-1-AP (same clone as 12945-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12945-1-AP (GAGE7 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12945-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 12945-1-AP (same clone as 12945-1-PBS)

    IHC staining of human prostate cancer using 12945-1-AP (same clone as 12945-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12945-1-AP (GAGE7 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12945-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 12945-1-AP (same clone as 12945-1-PBS)

    IHC staining of human prostate cancer using 12945-1-AP (same clone as 12945-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12945-1-AP (GAGE7 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12945-1-PBS in a different storage buffer formulation.

GAGE7 Polyclonal antibody, PBS Only

GAGE7 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12945-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

AL4, Cancer/testis antigen 4.7, CT4.7, G antigen 12F, G antigen 7

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12945-1-PBS targets GAGE7 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4018

Product name: Recombinant human GAGE7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-117 aa of BC031628

Sequence: MSWRGRSTYYWPRPRRYVQPPEMIGPMRPEQFSDEVEPATPEEGEPATQRQDPAAAQEGEDEGASAGQGPKPEAHSQEQGHPQTGCECEDGPDGQEMDPPNPEEVKTPEEGEKQSQC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G antigen 7
别名 AL4, Cancer/testis antigen 4.7, CT4.7, G antigen 12F, G antigen 7
计算分子量 13 kDa
观测分子量26 kDa
GenBank蛋白编号BC031628
基因名称 GAGE7
Gene ID (NCBI) 2579
RRIDAB_2877895
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO76087
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GAGE7 (G Antigen 7), also known as CT4.7 or GAGE-7, is a cancer-testis antigen belonging to the GAGE family. It is predominantly expressed in germ cells of the testis and various tumors, including melanoma, lung, and breast cancers, while remaining silent in normal adult tissues (PMID: 10397259). GAGE7 is an intrinsically disordered protein (IDP) of 117 amino acids with a theoretical molecular weight of ~13 kDa; however, it exhibits an apparent molecular weight of ~26 kDa in SDS-PAGE due to its abnormal electrophoretic mobility (PMID: 23029259).

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