• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 12945-1-AP

    WB analysis using 12945-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 12945-1-AP (GAGE7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of LNCaP using 12945-1-AP

    WB analysis of LNCaP using 12945-1-AP

    WB result of GAGE7 antibody (12945-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-GAGE7 transfected LNCaP cells.

  • IHC staining of human gliomas using 12945-1-AP

    IHC staining of human gliomas using 12945-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12945-1-AP (GAGE7 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human gliomas using 12945-1-AP

    IHC staining of human gliomas using 12945-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12945-1-AP (GAGE7 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human prostate cancer using 12945-1-AP

    IHC staining of human prostate cancer using 12945-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12945-1-AP (GAGE7 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human prostate cancer using 12945-1-AP

    IHC staining of human prostate cancer using 12945-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12945-1-AP (GAGE7 Antibody) at dilution of 1:50 (under 40x lens).

GAGE7 Polyclonal antibody

GAGE7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12945-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

AL4, Cancer/testis antigen 4.7, CT4.7, G antigen 12F, G antigen 7

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inLNCaP cells, BGC-823 cells, DU 145 cells, HGC-27 cells, MKN-45 cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12945-1-AP targets GAGE7 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag4018

Product name: Recombinant human GAGE7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-117 aa of BC031628

Sequence: MSWRGRSTYYWPRPRRYVQPPEMIGPMRPEQFSDEVEPATPEEGEPATQRQDPAAAQEGEDEGASAGQGPKPEAHSQEQGHPQTGCECEDGPDGQEMDPPNPEEVKTPEEGEKQSQC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G antigen 7
别名 AL4, Cancer/testis antigen 4.7, CT4.7, G antigen 12F, G antigen 7
计算分子量 13 kDa
观测分子量26 kDa
GenBank蛋白编号BC031628
基因名称 GAGE7
Gene ID (NCBI) 2579
RRIDAB_2877895
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO76087
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAGE7 (G Antigen 7), also known as CT4.7 or GAGE-7, is a cancer-testis antigen belonging to the GAGE family. It is predominantly expressed in germ cells of the testis and various tumors, including melanoma, lung, and breast cancers, while remaining silent in normal adult tissues (PMID: 10397259). GAGE7 is an intrinsically disordered protein (IDP) of 117 amino acids with a theoretical molecular weight of ~13 kDa; however, it exhibits an apparent molecular weight of ~26 kDa in SDS-PAGE due to its abnormal electrophoretic mobility (PMID: 23029259).

实验方案

Product Specific Protocols
IHC protocol for GAGE7 antibody 12945-1-APDownload protocol
WB protocol for GAGE7 antibody 12945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

J Cancer Res Clin Oncol

Elevated ZBTB7A expression in the tumor invasive front correlates with more tumor budding formation in gastric adenocarcinoma.

Authors - Yujing Sun
View Article
humanWB,IHC

J Exp Clin Cancer Res

GAGE7B promotes tumor metastasis and growth via activating the p38δ/pMAPKAPK2/pHSP27 pathway in gastric cancer.

Authors - Duan-Bo Shi
View Article
||
New chat

Able™

正在加载，请稍候...