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验证数据展示

  • IHC staining of human stomach cancer using 84376-3-RR

    IHC staining of human stomach cancer using 84376-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84376-3-RR (GADD45A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 84376-3-RR

    IHC staining of human stomach cancer using 84376-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84376-3-RR (GADD45A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 84376-3-RR

    IF Staining of U2OS using 84376-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GADD45A antibody (84376-3-RR, Clone: 241597F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of K-562 using 84376-3-RR

    FC experiment of K-562 using 84376-3-RR

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug GADD45A Recombinant antibody (84376-3-RR, Clone:241597F5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84376-3-RR

    Affinity and Kinetic Characterization of 84376-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84376-3-RR against Human GADD45A were performed. The affinity constant is below 1 pM.

GADD45A Recombinant antibody

GADD45A Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84376-3-RR

产品说明书

CloneNo. 241597F5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

241597F5, DDIT 1, DDIT1, DDIT-1, DNA damage-inducible transcript 1 protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inK-562 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84376-3-RR targets GADD45A in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 GADD45A fusion protein Ag4687 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 growth arrest and DNA-damage-inducible, alpha
别名 241597F5, DDIT 1, DDIT1, DDIT-1, DNA damage-inducible transcript 1 protein
计算分子量 165 aa, 18 kDa
GenBank蛋白编号BC011757
基因名称 GADD45A
Gene ID (NCBI) 1647
RRIDAB_3671912
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP24522
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for GADD45A antibody 84376-3-RRDownload protocol
IF protocol for GADD45A antibody 84376-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols