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验证数据展示

  • WB analysis using 67648-1-Ig (same clone as 67648-1-PBS)

    WB analysis using 67648-1-Ig (same clone as 67648-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

  • IHC staining of rat cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

    IHC staining of rat cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GAD1 antibody included
  • IHC staining of mouse cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

    IHC staining of mouse cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GAD1 antibody included
  • IF Staining of rat brain using 67648-1-Ig (same clone as 67648-1-PBS)

    IF Staining of rat brain using 67648-1-Ig (same clone as 67648-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GAD1 antibody (67648-1-Ig, Clone: 2D5B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

GAD1 Monoclonal antibody, PBS Only

GAD1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67648-1-PBS

产品说明书

CloneNo. 2D5B7

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF-P, Indirect ELISA

别名

GAD67, 2D5B7, GAD, GAD 1, GAD 67

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GAD1 Ready-To-Use IHC Kit. GAD1 primary antibody included.

产品信息

67648-1-PBS targets GAD1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag21336

Product name: Recombinant human GAD1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-224 aa of BC002815

Sequence: MASSTPSSSATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFKERQSSKNLLSCENSDRDARFRRTETDFSNLFARDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMPSDMRECWLLR

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 glutamate decarboxylase 1 (brain, 67kDa)
别名 GAD67, 2D5B7, GAD, GAD 1, GAD 67
计算分子量 67 kDa
观测分子量 67 kDa, 57 kDa
GenBank蛋白编号BC002815
基因名称 GAD1
Gene ID (NCBI) 2571
RRIDAB_2882848
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ99259
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GAD1, also named as GAD67, belongs to the group II decarboxylase family. GAD1 is the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities.

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