验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue, rat brain tissue, mouse cerebellum tissue, pig cerebellum tissue, rabbit brain tissue, rabbit cerebellum tissue, rat cerebellum tissue, mouse brain tissue |
| Positive IHC detected in | rat cerebellum tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67648-1-Ig targets GAD1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag21336 Product name: Recombinant human GAD1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-224 aa of BC002815 Sequence: MASSTPSSSATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFKERQSSKNLLSCENSDRDARFRRTETDFSNLFARDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMPSDMRECWLLR 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | glutamate decarboxylase 1 (brain, 67kDa) |
| 别名 | 2D5B7, EC:4.1.1.15, FLJ45882, GAD, GAD 1 |
| 计算分子量 | 67 kDa |
| 观测分子量 | 67 kDa, 57 kDa |
| GenBank蛋白编号 | BC002815 |
| 基因名称 | GAD1 |
| Gene ID (NCBI) | 2571 |
| RRID | AB_2882848 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q99259 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GAD1, also named as GAD67, belongs to the group II decarboxylase family. GAD1 is the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GAD1 antibody 67648-1-Ig | Download protocol |
| IHC protocol for GAD1 antibody 67648-1-Ig | Download protocol |
| WB protocol for GAD1 antibody 67648-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biol Trace Elem Res Effect of Voluntary Wheel Running on Anxiety- and Depression-Like Behaviors in Fluoride-Exposed Mice | ||
Acta Histochem Proposal for a simple and easy-to-implement protocol for three-dimensional tissue imaging that is compatible with observation using a confocal microscope | ||
Behav Brain Res Targeted suppression of BRD7 with BI-9564 prevents seizure behaviors in pentylenetetrazol and pilocarpine-induced mouse model of epilepsy | ||
CNS Neurosci Ther Downregulation of Mfn2 Contributes to Chronic Postsurgical Pain via Inducing the Pyroptosis of GABAergic Neurons in the Spinal Cord | ||
Dev Cell The de novo synthesis of GABA and its gene regulatory function control hepatocellular carcinoma metastasis | ||
Acta Pharmacol Sin Differential regulation of pruritic sensation and emotion by cannabinoid type 1 receptors on mPFC glutamatergic and GABAergic neurons |
