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验证数据展示

  • WB analysis using 66860-1-Ig (same clone as 66860-1-PBS)

    WB analysis using 66860-1-Ig (same clone as 66860-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66860-1-Ig (G6PC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66860-1-Ig (same clone as 66860-1-PBS)

    IHC staining of human liver using 66860-1-Ig (same clone as 66860-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66860-1-Ig (G6PC antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66860-1-Ig (same clone as 66860-1-PBS)

    IHC staining of human liver using 66860-1-Ig (same clone as 66860-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66860-1-Ig (G6PC antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

  • WB analysis of HuH-7 using 66860-1-Ig (same clone as 66860-1-PBS)

    WB analysis of HuH-7 using 66860-1-Ig (same clone as 66860-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 66860-1-Ig (G6PC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 66860-1-Ig (same clone as 66860-1-PBS)

    IF Staining of mouse liver using 66860-1-Ig (same clone as 66860-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using G6PC antibody (66860-1-Ig, Clone: 1E11A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66860-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50826-2

    Cytometric bead array standard curve of MP50826-2

    Cytometric bead array standard curve of MP50826-2, G6PC Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66860-4-PBS. Detection antibody: 66860-1-PBS. Standard:Ag17839. Range: 0.098-100 ng/mL.

G6PC Monoclonal antibody, PBS Only (Detector)

G6PC Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 66860-1-PBS

产品说明书

CloneNo. 1E11A5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

G6PC1, 1E11A5, EC:3.1.3.9, G6Pase, G-6-Pase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66860-1-PBS targets G6PC as part of a matched antibody pair:

MP50826-2: 66860-4-PBS capture and 66860-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag17839

Product name: Recombinant human G6PC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-60 aa of BC130478

Sequence: MEEGMNVLHDFGIQSTHYLQVNYQDSQDWFILVSVIADLRNAFYVLFPIWFHLQEAVGIK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glucose-6-phosphatase, catalytic subunit
别名 G6PC1, 1E11A5, EC:3.1.3.9, G6Pase, G-6-Pase
计算分子量 357 aa, 40 kDa
观测分子量 37-42 kDa
GenBank蛋白编号BC130478
基因名称 G6PC
Gene ID (NCBI) 2538
RRIDAB_2882199
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP35575
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glucose-6-phosphatase-α (G6PC) is a key enzyme in glucose homeostasis that catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the terminal step of gluconeogenesis and glycogenolysis. G6PC activity is restricted to the liver , the kidney cortex and the small intestine and confers on these three organs the capacity to release glucose into the systemic circulation (PMID: 21983240).The encoded enzyme is anchored to the ER by nine transmembrane helices with the amino (N)-terminus in the lumen and the carboxyl (C)-terminus in the cytoplasm (PMID: 15542400).