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G6PC Monoclonal antibody

G6PC Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E11A5

Cat No : 66860-1-Ig

Print datasheet

Synonyms

G 6 Pase, G6P, G6Pase, G6Pase alpha, G6PC, G6PT, Glucose 6 phosphatase, Glucose 6 phosphatase alpha, GSD1, GSD1a

验证数据展示

  • WB analysis using 66860-1-Ig

    WB analysis using 66860-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66860-1-Ig (G6PC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human liver using 66860-1-Ig

    IHC staining of human liver using 66860-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66860-1-Ig (G6PC antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 66860-1-Ig

    IHC staining of human liver using 66860-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66860-1-Ig (G6PC antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HuH-7 using 66860-1-Ig

    WB analysis of HuH-7 using 66860-1-Ig

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 66860-1-Ig (G6PC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHuH-7 cells, L02 cells, HepG2 cells
Positive IHC detected inhuman liver tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

66860-1-Ig targets G6PC in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen G6PC fusion protein Ag17839 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glucose-6-phosphatase, catalytic subunit
Synonyms G 6 Pase, G6P, G6Pase, G6Pase alpha, G6PC, G6PT, Glucose 6 phosphatase, Glucose 6 phosphatase alpha, GSD1, GSD1a
Calculated Molecular Weight 357 aa, 40 kDa
Observed Molecular Weight 37-42 kDa
GenBank Accession NumberBC130478
Gene Symbol G6PC
Gene ID (NCBI) 2538
RRIDAB_2882199
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35575
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glucose-6-phosphatase-α (G6PC) is a key enzyme in glucose homeostasis that catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the terminal step of gluconeogenesis and glycogenolysis. G6PC activity is restricted to the liver , the kidney cortex and the small intestine and confers on these three organs the capacity to release glucose into the systemic circulation (PMID: 21983240).The encoded enzyme is anchored to the ER by nine transmembrane helices with the amino (N)-terminus in the lumen and the carboxyl (C)-terminus in the cytoplasm (PMID: 15542400).

实验方案

Product Specific Protocols
WB protocol for G6PC antibody 66860-1-IgDownload protocol
IHC protocol for G6PC antibody 66860-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Pharmacol

Berberrubine, a Main Metabolite of Berberine, Alleviates Non-Alcoholic Fatty Liver Disease via Modulating Glucose and Lipid Metabolism and Restoring Gut Microbiota

Authors - Sa Yang
View Article
humanWB

Front Cell Dev Biol

Single-cell and genetic multi-omics analysis combined with experiments confirmed the signature and potential targets of cuproptosis in hepatocellular carcinoma

Authors - Feng Cao
View Article