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CoraLite® Plus 488-conjugated Follistatin Monoclonal antibody

Follistatin Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4E9B10

Cat No : CL488-60060

Print datasheet

Synonyms

Activin binding protein, follistatin, FS, FST

验证数据展示

  • IF Staining of HeLa using CL488-60060

    IF Staining of HeLa using CL488-60060

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Follistatin antibody (CL488-60060, Clone: 4E9B10 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60060-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60060 targets Follistatin in IF applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse
Immunogen Follistatin fusion protein Ag0775 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name follistatin
Synonyms Activin binding protein, follistatin, FS, FST
Calculated Molecular Weight 33 aa, 7 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC004107
Gene Symbol FST
Gene ID (NCBI) 10468
RRIDAB_2883093
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodCaprylic Acid-Ammonium Sulfate Precipitation
UNIPROT IDP19883
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Follistatin (FST) is a member of the tissue growth factor β family and is a secreted glycoprotein that antagonizes many members of the family, including activin A, growth differentiation factor11 , and myostatin. It binds activin A with high affinity and whose expression can be induced by activin A and several other pro-inflammatory cytokines. Activin A-follistatin complexes are biologically inactive and bind to cell surface heparan sulphate-containing proteoglycans for internalisation and degradation. It has also been found to play a significant role in the management of skeletal muscle size and mass. It also has important roles in early embryonic development, differentiation of ovarian granulosa cells, liver fibrosis and polycystic ovarian syndrome.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Follistatin antibody CL488-60060Download protocol
Standard Protocols
Click here to view our Standard Protocols