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验证数据展示

  • WB analysis using 32442-1-AP (same clone as 32442-1-PBS)

    WB analysis using 32442-1-AP (same clone as 32442-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32442-1-AP (FCN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32442-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 32442-1-AP (same clone as 32442-1-PBS)

    IF Staining of mouse liver using 32442-1-AP (same clone as 32442-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Ficolin 2 antibody (32442-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32442-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 32442-1-AP (same clone as 32442-1-PBS)

    IHC staining of mouse liver using 32442-1-AP (same clone as 32442-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32442-1-AP (Ficolin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32442-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 32442-1-AP (same clone as 32442-1-PBS)

    IHC staining of mouse liver using 32442-1-AP (same clone as 32442-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32442-1-AP (Ficolin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32442-1-PBS in a different storage buffer formulation.

Ficolin 2 Polyclonal antibody, PBS Only

Ficolin 2 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 32442-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

FCN2, FCNL, Ficolin B, Ficolin beta, Hucolin

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

32442-1-PBS targets Ficolin 2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag37525

Product name: Recombinant human FCN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 228-288 aa of NM_004108

Sequence: EGSAGDSLTFHNNQSFSTKDQDNDLNTGNCAVMFQGAWWYKNCHVSNLNGRYLRGTHGSFA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin)
别名 FCN2, FCNL, Ficolin B, Ficolin beta, Hucolin
计算分子量34 kDa
观测分子量34-35 kDa
GenBank蛋白编号NM_004108
基因名称 FCN2
Gene ID (NCBI) 2220
RRIDAB_3742612
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ15485
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ficolins are a group of oligomeric lectins with subunits consisting of both collagen-like and fibrinogen-like domains (PMID: 20375620). They are innate pattern recognition receptors and play integral roles in the immune response. In humans, there are three ficolins termed M-, L-, and H-ficolin (also referred to as ficolin-1, -2, and -3) whereas rodents only possess ficolin-A and -B, which are the orthologues of human L- and M-ficolin, respectively (PMID: 30868077). Ficolin 2 recognizes pathogen-associated pattern molecules (PAMPs) on the surface of pathogenic microorganisms, activates the complement lectin pathway, and binds to mannose-binding lectin-associated serine proteases (MASPs) to exert immune defense function (PMID: 21315829).

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