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验证数据展示

  • WB analysis of MCF-7 using 60081-1-Ig (same clone as 60081-1-PBS)

    WB analysis of MCF-7 using 60081-1-Ig (same clone as 60081-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 60081-1-Ig (same clone as 60081-1-PBS)

    WB analysis of MCF-7 using 60081-1-Ig (same clone as 60081-1-PBS)

    WB result of Fibulin 5 antibody (60081-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Fibulin 5 transfected MCF-7 cells. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 60081-1-Ig (same clone as 60081-1-PBS)

    IF Staining of A549 using 60081-1-Ig (same clone as 60081-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed A549 cells using 60081-1-Ig(Fibulin 5 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Fibulin 5 Monoclonal antibody, PBS Only

Fibulin 5 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 60081-1-PBS

产品说明书

CloneNo. 2A5C8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IF/ICC, ELISA

别名

ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, FLJ90059, UP50, Urine p50 protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60081-1-PBS targets Fibulin 5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Fibulin 5 fusion protein Ag2859 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 fibulin 5
别名 ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, FLJ90059, UP50, Urine p50 protein
计算分子量 448 aa, 50 kDa
观测分子量 65 kDa
GenBank蛋白编号BC022280
基因名称 Fibulin 5
Gene ID (NCBI) 10516
RRIDAB_2231920
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UBX5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies.