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- KD/KO Validated
Fibulin 5 Monoclonal antibody
Fibulin 5 Monoclonal Antibody for IF/ICC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
IF/ICC, WB,ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
2A5C8
验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, pig heart tissue, human placenta tissue, human heart tissue, rat heart tissue |
| Positive IF detected in | A549 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF) | IF : 1:50-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60081-1-Ig targets Fibulin 5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | IF/ICC, WB,ELISA |
| Cited Applications | WB, IP, IF, IHC |
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Immunogen | Fibulin 5 fusion protein Ag2859 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | fibulin 5 |
| Synonyms | ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, UP50, Urine p50 protein |
| Calculated Molecular Weight | 448 aa, 50 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC022280 |
| Gene Symbol | FBLN5 |
| Gene ID (NCBI) | 10516 |
| RRID | AB_2231920 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Caprylic acid/ammonium sulfate precipitation |
| UNIPROT ID | Q9UBX5 |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Fibulin 5 antibody 60081-1-Ig | Download protocol |
| IF protocol for Fibulin 5 antibody 60081-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility. | ||
Mater Sci Eng C Mater Biol Appl Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation. | ||
PLoS One Oncogenic Fibulin-5 Promotes Nasopharyngeal Carcinoma Cell Metastasis through the FLJ10540/AKT Pathway and Correlates with Poor Prognosis. | ||
J Biomol Screen A high-throughput cell-based gaussia luciferase reporter assay for identifying modulators of fibulin-3 secretion. | ||
Stem Cells Int Effects of Fibulin-5 Gene Silencing on Proliferation and Apoptosis of IgG4-ROD Lacrimal Gland Fibroblasts | ||
Free Radic Biol Med Extracellular SOD modulates canonical TNFα signaling and α5β1 integrin transactivation in vascular smooth muscle cells
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