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验证数据展示

  • FC experiment of MSCs(human mesenchymal stem cells) using 98652-2-RR

    FC experiment of MSCs(human mesenchymal stem cells) using 98652-2-RR

    1x10^6 MSCs(human mesenchymal stem cells) cells were stained with 0.25 ug Anti-Human Fibronectin Rabbit RecAb (98652-2-RR, Clone:251989E9) (red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • IHC staining of human colon using 98652-2-RR

    IHC staining of human colon using 98652-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 98652-2-RR (Fibronectin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 98652-2-RR

    IHC staining of human colon using 98652-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 98652-2-RR (Fibronectin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NIH/3T3 using 98652-2-RR

    IF Staining of NIH/3T3 using 98652-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Fibronectin antibody (98652-2-RR, Clone: 251989E9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 98652-2-RR

    Affinity of 98652-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98652-2-RR against Human Fibronectin were performed. The affinity constant is 94.5 pM.

Anti-Human Fibronectin Rabbit Recombinant Antibody

Fibronectin Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC(intra)
Cat No. 98652-2-RR

产品说明书

CloneNo. 251989E9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC(intra)

别名

FN1, Anastellin, CIG, Cold insoluble globulin, Cold-insoluble globulin

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Positive FC(intra) detected inMSCs (human mesenchymal stem cells)

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC)(INTRA)FC(INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98652-2-RR targets Fibronectin in IHC, IF/ICC, FC(intra) applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC(intra) Application Description
经测试反应性 human
免疫原

CatNo: Eg5117

Product name: Recombinant Human Fibronectin protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 607-1265 aa of NM_212482.4

Sequence: SSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fibronectin 1
别名 FN1, Anastellin, CIG, Cold insoluble globulin, Cold-insoluble globulin
计算分子量272 kDa
GenBank蛋白编号NM_212482.4
基因名称 Fibronectin
Gene ID (NCBI) 2335
RRIDAB_3746249
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02751-15
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)

实验方案

Product Specific Protocols
FC protocol for Fibronectin antibody 98652-2-RRDownload protocol
IF protocol for Fibronectin antibody 98652-2-RRDownload protocol
IHC protocol for Fibronectin antibody 98652-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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