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验证数据展示

  • WB analysis using 80911-4-RR

    WB analysis using 80911-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80911-4-RR (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 80911-4-RR

    WB analysis of NIH/3T3 using 80911-4-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80911-4-RR (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat plasma using 80911-4-RR

    WB analysis of rat plasma using 80911-4-RR

    rat plasma were subjected to SDS PAGE followed by western blot with 80911-4-RR (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 80911-4-RR

    IF Staining of NIH/3T3 using 80911-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Fibronectin antibody (80911-4-RR, Clone: 251989E12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Fibronectin Recombinant monoclonal antibody

Fibronectin Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 80911-4-RR

产品说明书

CloneNo. 251989E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

FN1, Anastellin, CIG, Cold insoluble globulin, Cold-insoluble globulin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, HepG2 cells, rat plasma, mouse uterus tissue
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80911-4-RR targets Fibronectin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg5117

Product name: Recombinant Human Fibronectin protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 607-1265 aa of NM_212482.4

Sequence: SSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fibronectin 1
别名 FN1, Anastellin, CIG, Cold insoluble globulin, Cold-insoluble globulin
计算分子量272 kDa
观测分子量220-275 kDa
GenBank蛋白编号NM_212482.4
基因名称 Fibronectin
Gene ID (NCBI) 2335
RRIDAB_3743358
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02751-15
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibronectin (fibronectin) is a kind of macromolecular glycoprotein widely existing in extracellular matrix (ECM), plasma and various cell surfaces, which plays a central role in key biological processes such as cell adhesion, migration, proliferation, differentiation, tissue repair and embryo development. Fibronectin is usually a dimer structure, which consists of two subunits connected by disulfide bonds. There are two main forms of Fibronectin: plasma Fibronectin(pFN) is synthesized by liver cells and exists in the blood, which is involved in the processes of coagulation and wound repair. Cellular Fibronectin(cFN) is synthesized by fibroblasts and other cells, deposited in extracellular matrix, and participates in the interaction between cells and matrix. Its molecular weight ranges from 220 to 275 kDa.

实验方案

Product Specific Protocols
IF protocol for Fibronectin antibody 80911-4-RRDownload protocol
WB protocol for Fibronectin antibody 80911-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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