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验证数据展示

  • WB analysis using 86913-6-RR

    WB analysis using 86913-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86913-6-RR (FGA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 86913-6-RR

    IHC staining of human kidney using 86913-6-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86913-6-RR (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 86913-6-RR

    IHC staining of human placenta using 86913-6-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86913-6-RR (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Fibrinogen Alpha Chain Recombinant monoclonal antibody

Fibrinogen Alpha Chain Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 86913-6-RR

产品说明书

CloneNo. 260891B12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

FGA, Fib2, Fibrinopeptide A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman placenta tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86913-6-RR targets Fibrinogen Alpha Chain in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33674

Product name: Recombinant human FGA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 20-218 aa of BC020764

Sequence: ADSGEGDFLAEGGGVRGPRVVERHQSACKDSDWPFCSGEDWNYKCPSGCRMKGLIDEVNQDFTNRINKLKNSLFEYQKNNKDSHSLTTNIMEILRGDFSSANNRDNTYNRVSEDLRSRIEVLKRKVIEKVTANNLLVARVTTEETPHLKARAIKWQMRPEVKPIMKEHIAPREAMLNLALSEVSTLLLWGSLPCPPRLS*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fibrinogen alpha chain
别名 FGA, Fib2, Fibrinopeptide A
计算分子量 866 aa, 95 kDa
观测分子量95kDa, 70 kDa
GenBank蛋白编号BC020764
基因名称 Fibrinogen Alpha Chain
Gene ID (NCBI) 2243
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02671
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGA is the alpha chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide A is cleaved from FGA by thrombin.

实验方案

Product Specific Protocols
IHC protocol for Fibrinogen Alpha Chain antibody 86913-6-RRDownload protocol
WB protocol for Fibrinogen Alpha Chain antibody 86913-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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