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验证数据展示

  • WB analysis of MC/9 using 84662-4-RR

    WB analysis of MC/9 using 84662-4-RR

    MC/9 cells were subjected to SDS PAGE followed by western blot with 84662-4-RR (Fc epsilon RI alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 84662-4-RR

    WB analysis of human testis using 84662-4-RR

    human testis tissue were subjected to SDS PAGE followed by western blot with 84662-4-RR (FceR1a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity of 84662-4-RR

    Affinity of 84662-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84662-4-RR against Human FceR1a were performed. The affinity constant is below 1 pM.

FceR1a Recombinant monoclonal antibody

FceR1a Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 84662-4-RR

产品说明书

CloneNo. 241848D6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

Fc epsilon RI alpha, FCE1A, Fc-epsilon RI-alpha, FcERI, FceRIa

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMC/9 cells, human testis tissue, KU812 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84662-4-RR targets FceR1a in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg2022

Product name: Recombinant Human Fc Epsilon RI Alpha protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 26-205 aa of NM_001387280.1

Sequence: VPQKPKVSLNPPWNRIFKGENVTLTCNGNNFFEVSSTKWFHNGSLSEETNSSLNIVNAKFEDSGEYKCQHQQVNESEPVYLEVFSDWLLLQASAEVVMEGQPLFLRCHGWRNWDVYKVIYYKDGEALKYWYENHNISITNATVEDSGTYYCTGKVWQLDYESEPLNITVIKAPREKYWLQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
别名 Fc epsilon RI alpha, FCE1A, Fc-epsilon RI-alpha, FcERI, FceRIa
计算分子量30 kDa
观测分子量45-55 kDa
GenBank蛋白编号NM_001387280.1
基因名称 FceR1a
Gene ID (NCBI) 2205
RRIDAB_3743641
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP12319
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fc epsilon Receptor I alpha (FceR1a) is a single-pass type I membrane protein that contains 2 immunoglobulin-like domains. FceR1a forms a tetrameric complex, FceRI, with one beta and two gamma subunits (PMID: 19406478). FceRI is the high-affinity receptor for the Fc region of immunoglobulin E (IgE) (PMID: 1532373). It is expressed on mast cells and basophils and plays a central role in the allergic response (PMID: 2148316). The calculated molecular weight of FceR1a is 30 kDa, the 45- to 55-kDa bands detected by this antibody are probably caused by heterogeneous glycosylation.

实验方案

Product Specific Protocols
WB protocol for FceR1a antibody 84662-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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