验证数据展示
经过测试的应用
Positive WB detected in | human testis tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84662-3-RR targets FceR1a in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human |
免疫原 | Recombinant protein 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide |
别名 | FceRIa, FcERI, Fc-epsilon RI-alpha, FCE1A, Fc epsilon RI alpha |
计算分子量 | 30kDa |
观测分子量 | 55-65 kDa |
GenBank蛋白编号 | NM_001387280.1 |
基因名称 | FceR1a |
Gene ID (NCBI) | 2205 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P12319 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fc epsilon Receptor I alpha (FceR1a) is a single-pass type I membrane protein that contains 2 immunoglobulin-like domains. FceR1a forms a tetrameric complex, FceRI, with one beta and two gamma subunits (PMID: 19406478). FceRI is the high-affinity receptor for the Fc region of immunoglobulin E (IgE) (PMID: 1532373). It is expressed on mast cells and basophils and plays a central role in the allergic response (PMID: 2148316). The calculated molecular weight of FceR1a is 30 kDa, the 55- to 70-kDa bands detected by this antibody are probably caused by heterogeneous glycosylation (PMID: 11344350; 12671054).
实验方案
Product Specific Protocols | |
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WB protocol for FceR1a antibody 84662-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |