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CoraLite® Plus 488-conjugated Fascin Monoclonal antibody

Fascin Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B5G3

Cat No : CL488-66321

Print datasheet

Synonyms

55 kDa actin bundling protein, FAN1, Fascin, Fascin 1,Fascin, FLJ38511, FSCN1, HSN, p55, Singed like protein, SNL

验证数据展示

  • IF Staining of human tonsillitis using CL488-66321

    IF Staining of human tonsillitis using CL488-66321

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66321 (Fascin antibody) at dilution of 1:100.

  • IF Staining of human tonsillitis using CL488-66321

    IF Staining of human tonsillitis using CL488-66321

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66321 (Fascin antibody) at dilution of 1:100. .

  • FC experiment of SH-SY5Y using CL488-66321

    FC experiment of SH-SY5Y using CL488-66321

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Fascin (CL488-66321, Clone:1B5G3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inhuman tonsillitis tissue
Positive FC detected inSH-SY5Y cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy Fascin Ready-To-Use IHC Kit. Fascin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66321-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66321 targets Fascin in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human
Immunogen Fascin fusion protein Ag6074 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
Synonyms 55 kDa actin bundling protein, FAN1, Fascin, Fascin 1,Fascin, FLJ38511, FSCN1, HSN, p55, Singed like protein, SNL
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC000521
Gene Symbol FSCN1
Gene ID (NCBI) 6624
ENSEMBL Gene IDENSG00000075618
RRIDAB_2883308
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16658
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Fascin antibody CL488-66321Download protocol
FC protocol for CL Plus 488 Fascin antibody CL488-66321Download protocol
Standard Protocols
Click here to view our Standard Protocols