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验证数据展示

  • FC experiment of human PBMCs using Biotin-FcA98034

    FC experiment of human PBMCs using Biotin-FcA98034

    1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human Fas/CD95 Rabbit RecAb (Biotin-FcA98034, Clone: 240340E11) and PE Streptavidin (Cat.No. PF00021), or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) and PE Streptavidin(Cat.No. PF00021). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD45RO (UCHL1) (CL647-65150, Clone: UCHL1). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using Biotin-FcA98034

    FC experiment of human PBMCs using Biotin-FcA98034

    1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human Fas/CD95 Rabbit RecAb (Biotin-FcA98034, Clone: 240340E11) (red) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and PE Streptavidin. Cells were not fixed. Lymphocytes were gated.

  • Zero Fc receptor binding
  • Site-specific conjugation
  • One universal isotype

FcZero-rAb® Biotin Anti-Human Fas/CD95 Rabbit Recombinant Antibody

Fas/CD95 FcZero-rAb® Recombinant Antibody for FC, ELISA
Cat No. Biotin-FcA98034

产品说明书

CloneNo. 240340E11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC, ELISA

别名

FAS, ALPS1A, APO-1, Apo-1 antigen, Apoptosis-mediating surface antigen FAS

缓冲液配方:  PBS, Azide
PBS, Azide
偶联物:  Biotin
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-FcA98034 targets Fas/CD95 in FC, ELISA applications and shows reactivity with human samples.

经测试应用 FC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg0877

Product name: Recombinant Human Fas/CD95 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 26-173 aa of NM_000043.6

Sequence: QVTDINSKGLELRKTVTTVETQNLEGLHHDGQFCHKPCPPGERKARDCTVNGDEPDCVPCQEGKEYTDKAHFSSKCRRCRLCDEGHGLEVEINCTRTQNTKCRCKPNFFCNSTVCEHCDPCTKCEHGIIKECTLTSNTKCKEEGSRSN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fas (TNF receptor superfamily, member 6)
别名 FAS, ALPS1A, APO-1, Apo-1 antigen, Apoptosis-mediating surface antigen FAS
计算分子量38 kDa
GenBank蛋白编号NM_000043.6
基因名称 Fas/CD95
Gene ID (NCBI) 355
偶联类型 Biotin
最大激发/发射波长-
形式Liquid
纯化方式Protein A purification
UNIPROT IDP25445-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.

实验方案

Product Specific Protocols
FC protocol for Biotin Fas/CD95 antibody Biotin-FcA98034Download protocol
Standard Protocols
Click here to view our Standard Protocols
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