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验证数据展示

  • WB analysis using 25730-1-AP (same clone as 25730-1-PBS)

    WB analysis using 25730-1-AP (same clone as 25730-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25730-1-AP (FYCO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25730-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 25730-1-AP (same clone as 25730-1-PBS)

    WB analysis of HeLa using 25730-1-AP (same clone as 25730-1-PBS)

    WB result of FYCO1 antibody (25730-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FYCO1 transfected HeLa cells. This data was developed using the same antibody clone with 25730-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FYCO1 Polyclonal antibody, PBS Only

FYCO1 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 25730-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25730-1-PBS targets FYCO1 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22803

Product name: Recombinant human FYCO1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-250 aa of BC007218

Sequence: MNTKVTSDWYYARSPFLQPKLSSDIVGQLYELTEVQFDLASRGFDLDAAWPTFARRTLTTGSSAYLWKPPSRSSSMSSLVSSYLQTQEMVSNFDLNSPLNNEALEGFDEMRLELDQLEVREKQLQERMQQLDRENQELRAAVSQQGEQLQTERERGRTAAEDNVRLTCLVAELQKQWEVTQATQNTVKELQTCLQALELGAAEKEEDYHTALRRLESMLQPLAQELEATRDSLDKKNQHLASFPGWLAMA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FYVE and coiled-coil domain containing 1
别名
计算分子量 1478 aa, 167 kDa
观测分子量167-180 kDa
GenBank蛋白编号BC007218
基因名称 FYCO1
Gene ID (NCBI) 79443
RRIDAB_3085821
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQS8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FYCO1 is a Rab7 adapter protein that is implicated in the microtubule transport of autophagosomes. The encoded protein contains a RUN domain, a FYVE-type zinc finger domain, and Golgi dynamics (GOLD) domain. The encoded protein plays a role in microtubule plus end-directed transport of autophagic vesicles through interactions with the small GTPase Rab7, phosphatidylinositol-3-phosphate (PI3P), the autophagosome marker LC3, and the kinesin KIF5.

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