验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HeLa cells, LNCaP cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
25730-1-AP targets FYCO1 in WB, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag22803 Product name: Recombinant human FYCO1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-250 aa of BC007218 Sequence: MNTKVTSDWYYARSPFLQPKLSSDIVGQLYELTEVQFDLASRGFDLDAAWPTFARRTLTTGSSAYLWKPPSRSSSMSSLVSSYLQTQEMVSNFDLNSPLNNEALEGFDEMRLELDQLEVREKQLQERMQQLDRENQELRAAVSQQGEQLQTERERGRTAAEDNVRLTCLVAELQKQWEVTQATQNTVKELQTCLQALELGAAEKEEDYHTALRRLESMLQPLAQELEATRDSLDKKNQHLASFPGWLAMA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | FYVE and coiled-coil domain containing 1 |
| 别名 | FYCO1, RUFY3, ZFYVE7 |
| 计算分子量 | 1478 aa, 167 kDa |
| 观测分子量 | 167-180 kDa |
| GenBank蛋白编号 | BC007218 |
| 基因名称 | FYCO1 |
| Gene ID (NCBI) | 79443 |
| RRID | AB_3085821 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BQS8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FYCO1 is a Rab7 adapter protein that is implicated in the microtubule transport of autophagosomes. The encoded protein contains a RUN domain, a FYVE-type zinc finger domain, and Golgi dynamics (GOLD) domain. The encoded protein plays a role in microtubule plus end-directed transport of autophagic vesicles through interactions with the small GTPase Rab7, phosphatidylinositol-3-phosphate (PI3P), the autophagosome marker LC3, and the kinesin KIF5.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FYCO1 antibody 25730-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Rep LRBA deficiency impairs autophagy and contributes to enhanced antigen presentation and T-cell dysregulation | ||
Signal Transduct Target Ther Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1. |
