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验证数据展示

  • WB analysis using 25730-1-AP

    WB analysis using 25730-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25730-1-AP (FYCO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 25730-1-AP

    WB analysis of HeLa using 25730-1-AP

    WB result of FYCO1 antibody (25730-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FYCO1 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

FYCO1 Polyclonal antibody

FYCO1 Polyclonal Antibody for WB, ELISA
Cat No. 25730-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA and More (1)

别名

FYCO1, RUFY3, ZFYVE7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, LNCaP cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25730-1-AP targets FYCO1 in WB, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag22803

Product name: Recombinant human FYCO1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-250 aa of BC007218

Sequence: MNTKVTSDWYYARSPFLQPKLSSDIVGQLYELTEVQFDLASRGFDLDAAWPTFARRTLTTGSSAYLWKPPSRSSSMSSLVSSYLQTQEMVSNFDLNSPLNNEALEGFDEMRLELDQLEVREKQLQERMQQLDRENQELRAAVSQQGEQLQTERERGRTAAEDNVRLTCLVAELQKQWEVTQATQNTVKELQTCLQALELGAAEKEEDYHTALRRLESMLQPLAQELEATRDSLDKKNQHLASFPGWLAMA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FYVE and coiled-coil domain containing 1
别名 FYCO1, RUFY3, ZFYVE7
计算分子量 1478 aa, 167 kDa
观测分子量167-180 kDa
GenBank蛋白编号BC007218
基因名称 FYCO1
Gene ID (NCBI) 79443
RRIDAB_3085821
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQS8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FYCO1 is a Rab7 adapter protein that is implicated in the microtubule transport of autophagosomes. The encoded protein contains a RUN domain, a FYVE-type zinc finger domain, and Golgi dynamics (GOLD) domain. The encoded protein plays a role in microtubule plus end-directed transport of autophagic vesicles through interactions with the small GTPase Rab7, phosphatidylinositol-3-phosphate (PI3P), the autophagosome marker LC3, and the kinesin KIF5.

实验方案

Product Specific Protocols
WB protocol for FYCO1 antibody 25730-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO Rep

LRBA deficiency impairs autophagy and contributes to enhanced antigen presentation and T-cell dysregulation

Authors - Elena Sindram
View Article
IF, WB

Signal Transduct Target Ther

Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1.

Authors - Wenchang Xiao
View Article