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验证数据展示

  • FC experiment of U-251 using CL488-27707

    FC experiment of U-251 using CL488-27707

    1x10^6 U-251 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Ftsjd2 Polyclonal Antibody (CL488-27707)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of U2OS using CL488-27707

    IF Staining of U2OS using CL488-27707

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 FTSJD2 antibody (CL488-27707) at dilution of 1:100, CL594-phalloidin (red).

CoraLite® Plus 488-conjugated FTSJD2 Polyclonal antibody

FTSJD2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-27707

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

FtsJ methyltransferase domain-containing protein 2, EC:2.1.1.57, CMTR1, Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1, Cap1 2'O-ribose methyltransferase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU-251 cells
For other applications, we recommend the unconjugated version of this antibody, 27707-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27707 targets FTSJD2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 FTSJD2 fusion protein Ag26722 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FtsJ methyltransferase domain containing 2
别名 FtsJ methyltransferase domain-containing protein 2, EC:2.1.1.57, CMTR1, Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1, Cap1 2'O-ribose methyltransferase 1
计算分子量 835 aa, 95 kDa
观测分子量 96-100 kDa
GenBank蛋白编号BC031890
基因名称 FTSJD2
Gene ID (NCBI) 23070
RRIDAB_3672815
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N1G2
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cap1 2′-O-methylation is catalyzed by cap methyltransferase 1 (CMTR1). CMTR1, also called IFN-stimulated gene 95 kDa protein (ISG95) or FTSJD2, is elevated by a viral infection and required to promote IFN-mediated induction of ISGs and the antiviral response. High CMTR1 expression is associated with poor prognosis in patients with CRC (PMID: 37024465). CMTr1 binds ATP-dependent RNA DHX15 helicase and that this interaction, mediated by the G-patch domain of CMTr1, has an advantageous effect on CMTr1 activity towards highly structured RNA substrates (PMID: 30397098).). Human CMTR1 is a 95 kDa nuclear protein that has multiple domains, consisting of a G-patch domain, a RrmJ/FtsJ methyltransferase domain, a non-functional cap guanylyltransferase-like domain and a WW domain (PMID: 30312682).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FTSJD2 antibody CL488-27707Download protocol
FC protocol for CL Plus 488 FTSJD2 antibody CL488-27707Download protocol
Standard Protocols
Click here to view our Standard Protocols