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验证数据展示

  • WB analysis of MCF-7 using 66263-1-Ig

    WB analysis of MCF-7 using 66263-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66263-1-Ig (FRS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 66263-1-Ig

    IHC staining of human prostate cancer using 66263-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66263-1-Ig (FRS2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FRS2 antibody included
  • IHC staining of human prostate cancer using 66263-1-Ig

    IHC staining of human prostate cancer using 66263-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66263-1-Ig (FRS2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FRS2 antibody included
  • IF Staining of MCF-7 using 66263-1-Ig

    IF Staining of MCF-7 using 66263-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FRS2 antibody (66263-1-Ig, Clone: 4E10H12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

FRS2 Monoclonal antibody

FRS2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66263-1-Ig

产品说明书

CloneNo. 4E10H12

宿主/亚型

Mouse / IgG3

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

SNT, FRS2alpha, FRS2A, FGFR-signaling adaptor SNT, FGFR substrate 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy FRS2 Ready-To-Use IHC Kit. FRS2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66263-1-Ig targets FRS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19299

Product name: Recombinant human FRS2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-300 aa of BC021562

Sequence: MGSCCSCPDKDTVPDNHRNKFKVINVDDDGNELGSGIMELTDTELILYTRKRDSVKWHYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCARAEELFNMLQEIMQNNSINVVEEPVVERNNHQTELEVPRTPRTPTTPGFAAQNLPNGYPRYPSFGDASSHPSSRHPSVGSARLPSVGEESTHPLLVAEEQVHTYVNTTGVQEERKNRTSVHVPLEARVSNAESSTPKEEPSSIEDRDPQILLEPEGVKFVLGPTPVQKQLMEKEKLEQLGRDQVSGSGANNTEWDTGYDSDERRDAPS

种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 fibroblast growth factor receptor substrate 2
别名 SNT, FRS2alpha, FRS2A, FGFR-signaling adaptor SNT, FGFR substrate 2
计算分子量 60 kDa
观测分子量 68 kDa
GenBank蛋白编号BC021562
基因名称 FRS2
Gene ID (NCBI) 10818
RRIDAB_2881650
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8WU20
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) has an alternative name as SNT-1, it is an adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. FGF receptor substrates (FRS2 and FRS3) are key adaptor proteins that mediate FGF-FGFR signalling in benign as well as malignant tissue. FRS2 is a 508 amino-acid protein,which is phosphorylated on tyrosine residues. The molecular weight of non-phosphorylated FRS2 is 57-68 kDa, but phosphorylated FRS2 is 80-90 kDa. Phosphorylation of FRS2 is associated with activation of a number of MAP kinases. Allele-specific regulation of FGFR2 mRNA expression with a mildly increased breast cancer risk has been reported.

实验方案

Product Specific Protocols
IF protocol for FRS2 antibody 66263-1-IgDownload protocol
IHC protocol for FRS2 antibody 66263-1-IgDownload protocol
WB protocol for FRS2 antibody 66263-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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