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验证数据展示

  • WB analysis of human plasma using 13086-1-AP (same clone as 13086-1-PBS)

    WB analysis of human plasma using 13086-1-AP (same clone as 13086-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 13086-1-AP (FREM1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 13086-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 13086-1-AP (same clone as 13086-1-PBS)

    WB analysis of HepG2 using 13086-1-AP (same clone as 13086-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13086-1-AP (FREM1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 13086-1-PBS in a different storage buffer formulation.

  • IHC staining of human hepatocellular cancer using 13086-1-AP (same clone as 13086-1-PBS)

    IHC staining of human hepatocellular cancer using 13086-1-AP (same clone as 13086-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 13086-1-AP (FREM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13086-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 13086-1-AP (same clone as 13086-1-PBS)

    IF Staining of HepG2 using 13086-1-AP (same clone as 13086-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FREM1 antibody (13086-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13086-1-PBS in a different storage buffer formulation.

FREM1 Polyclonal antibody, PBS Only

FREM1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13086-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

C9orf143, C9orf145, C9orf154, FRAS1-related extracellular matrix protein 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13086-1-PBS targets FREM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3617

Product name: Recombinant human FREM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 412-710 aa of BC031064

Sequence: SSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSPRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYGCSRKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FRAS1 related extracellular matrix 1
别名 C9orf143, C9orf145, C9orf154, FRAS1-related extracellular matrix protein 1
计算分子量2179aa, 244 kDa, 149.3 kDa
观测分子量 40-43 kDa, 70-80 kDa
GenBank蛋白编号BC031064
基因名称 FREM1
Gene ID (NCBI) 158326
RRIDAB_2877915
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5H8C1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FREM1, C9orf143, C9orf145, C9orf154 and QBRICK, is an extracellular matrix protein that plays a role in epidermal differentiation and is required for epidermal adhesion during embryonic development. This antibody recognizes isoform 1 (244 kDa), isform 2 (80 kDa) and isoform 3 (43 kDa).

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