PSMA/GCPII Monoclonal antibody

PSMA/GCPII Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66678-1-Ig

产品说明书

CloneNo. 3G4E12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, ELISA

FOLH1, PSMA, 3G4E12, Cell growth-inhibiting gene 27 protein, EC:3.4.17.21

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inrat kidney tissue, LNCaP cells, mouse kidney tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman prostate cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate cancer tissue, PC-3 cells
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy PSMA/FOLH1 Ready-To-Use IHC Kit. PSMA/FOLH1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

66678-1-Ig targets PSMA/GCPII in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 PSMA/GCPII fusion protein Ag16594 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 folate hydrolase (prostate-specific membrane antigen) 1
别名 FOLH1, PSMA, 3G4E12, Cell growth-inhibiting gene 27 protein, EC:3.4.17.21
计算分子量 719 aa, 81 kDa
观测分子量 100-120 kDa
GenBank蛋白编号BC025672
基因名称 PSMA
Gene ID (NCBI) 2346
RRIDAB_2882032
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ04609
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PSMA/GCPII antibody 66678-1-IgDownload protocol
IHC protocol for PSMA/GCPII antibody 66678-1-IgDownload protocol
IF protocol for PSMA/GCPII antibody 66678-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

Cancer cell's neuroendocrine feature can be acquired through cell-cell fusion during cancer-neural stem cell interaction.

Authors - Liyuan Yin
humanWB

Am J Clin Exp Urol

Transmembrane prostatic acid phosphatase: a therapeutic target in advanced prostate cancer

Authors - Alexander Kirschenbaum
human

RSC Med Chem

Design, synthesis and biological evaluation of a novel PSMA-PI3K small molecule drug conjugate

Authors - Shouguo Peng
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