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验证数据展示

  • WB analysis using 30297-1-AP (same clone as 30297-1-PBS)

    WB analysis using 30297-1-AP (same clone as 30297-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 30297-1-AP (FLT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30297-1-PBS in a different storage buffer formulation.

  • WB analysis of THP-1 using 30297-1-AP (same clone as 30297-1-PBS)

    WB analysis of THP-1 using 30297-1-AP (same clone as 30297-1-PBS)

    WB result of FLT3 antibody (30297-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-FLT3 transfected THP-1 cells. This data was developed using the same antibody clone with 30297-1-PBS in a different storage buffer formulation.

  • IP experiment of THP-1 using 30297-1-AP (same clone as 30297-1-PBS)

    IP experiment of THP-1 using 30297-1-AP (same clone as 30297-1-PBS)

    IP result of anti-FLT3 (IP:30297-1-AP, 4ug; Detection:30297-1-AP 1:500) with THP-1 cells lysate 2720 ug. This data was developed using the same antibody clone with 30297-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 30297-1-AP (same clone as 30297-1-PBS)

    IF Staining of HEK-293 using 30297-1-AP (same clone as 30297-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using FLT3 antibody (30297-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30297-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FLT3 Polyclonal antibody, PBS Only

FLT3 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 30297-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, Indirect ELISA

别名

CD135, Fetal liver kinase-2, FL cytokine receptor, FLK2, FLK-2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30297-1-PBS targets FLT3 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag32898

Product name: Recombinant human FLT3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 683-810 aa of BC126350

Sequence: LSGPIYLIFEYCCYGDLLNYLRSKREKFHRTWTEIFKEHNFSFYPTFQSHPNSSMPGSREVQIHPDSDQISGLHGNSFHSEDEIEYENQKRLEEEEDLNVLTFEDLLCFAYQVAKGMEFLEFKSCVHR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fms-related tyrosine kinase 3
别名 CD135, Fetal liver kinase-2, FL cytokine receptor, FLK2, FLK-2
计算分子量112 kDa
观测分子量130 kDa, 150 kDa
GenBank蛋白编号BC126350
基因名称 FLT3
Gene ID (NCBI) 2322
RRIDAB_3086289
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP36888
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FLT3 (also known as CD135 or FLK2) is a tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and of dendritic cells. FLT3 was originally identified by its expression in hematopoietic stem/progenitor cells (PMID: 7507245). It is important for the normal development of hematopoietic stem/progenitor cells. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

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