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验证数据展示

  • WB analysis using 30297-1-AP

    WB analysis using 30297-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 30297-1-AP (FLT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of THP-1 using 30297-1-AP

    IP experiment of THP-1 using 30297-1-AP

    IP result of anti-FLT3 (IP:30297-1-AP, 4ug; Detection:30297-1-AP 1:500) with THP-1 cells lysate 2720 ug.

  • IF Staining of HEK-293 using 30297-1-AP

    IF Staining of HEK-293 using 30297-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using FLT3 antibody (30297-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

FLT3 Polyclonal antibody

FLT3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 30297-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

CD135, Fetal liver kinase-2, FL cytokine receptor, FLK2, FLK-2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTHP-1 cells
Positive IP detected inTHP-1 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30297-1-AP targets FLT3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag32898

Product name: Recombinant human FLT3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 683-810 aa of BC126350

Sequence: LSGPIYLIFEYCCYGDLLNYLRSKREKFHRTWTEIFKEHNFSFYPTFQSHPNSSMPGSREVQIHPDSDQISGLHGNSFHSEDEIEYENQKRLEEEEDLNVLTFEDLLCFAYQVAKGMEFLEFKSCVHR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fms-related tyrosine kinase 3
别名 CD135, Fetal liver kinase-2, FL cytokine receptor, FLK2, FLK-2
计算分子量112 kDa
观测分子量130 kDa, 150 kDa
GenBank蛋白编号BC126350
基因名称 FLT3
Gene ID (NCBI) 2322
RRIDAB_3086289
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP36888
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FLT3 (also known as CD135 or FLK2) is a tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and of dendritic cells. FLT3 was originally identified by its expression in hematopoietic stem/progenitor cells (PMID: 7507245). It is important for the normal development of hematopoietic stem/progenitor cells. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

实验方案

Product Specific Protocols
IF protocol for FLT3 antibody 30297-1-APDownload protocol
IP protocol for FLT3 antibody 30297-1-APDownload protocol
WB protocol for FLT3 antibody 30297-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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