验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-67039 targets Flightless I in IF applications and shows reactivity with Human, Mouse, Rat samples.
经测试应用 | IF Application Description |
经测试反应性 | Human, Mouse, Rat |
免疫原 | Flightless I fusion protein Ag26865 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | flightless I homolog (Drosophila) |
别名 | FLI, Fli1, FLII, FLIL, Protein flightless 1 homolog |
计算分子量 | 1269 aa, 145 kDa |
观测分子量 | 145-150 kDa |
GenBank蛋白编号 | BC025300 |
基因名称 | Flightless I |
Gene ID (NCBI) | 2314 |
RRID | AB_2920077 |
偶联类型 | CoraLite®594 Fluorescent Dye |
最大激发/发射波长 | 588 nm / 604 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q13045 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Flightless I (FliI) is the most evolutionarily conserved member of the gelsolin superfamily of proteins which are key regulators of actin filament assembly and turnover. FliI comprises an N-terminal leucine-rich repeat (LRR) domain which is not present in other gelsolin family members, and the LRR domain may enable interactions between FliI and other molecules involved in signal transduction, thereby spatially integrating signaling and actin remodeling functions. This protein was originally found in Drosophila and participates in the embryonic development, while mammalian FliI protein was involved in the regulation of wound repair,skin barrier development. Studies recently demonstrated that FliI protein associated with colorectal cancer, hepatocellular and prostate cancer (PMID:30091651; 28498392).
实验方案
Product Specific Protocols | |
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IF protocol for CL594 Flightless I antibody CL594-67039 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |