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CoraLite®594-conjugated Flightless I Monoclonal antibody

Flightless I Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F9C8

Cat No : CL594-67039

Print datasheet

Synonyms

FLI, Fli1, FLII, FLIL, Protein flightless 1 homolog

验证数据展示

  • IF Staining of HeLa using CL594-67039

    IF Staining of HeLa using CL594-67039

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated Flightless I antibody (CL594-67039, Clone: 2F9C8 ) at dilution of 1:100.

经过测试的应用

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67039-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67039 targets Flightless I in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC
Tested Reactivity Human, Mouse, Rat
Immunogen Flightless I fusion protein Ag26865 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name flightless I homolog (Drosophila)
Synonyms FLI, Fli1, FLII, FLIL, Protein flightless 1 homolog
Calculated Molecular Weight 1269 aa, 145 kDa
Observed Molecular Weight 145-150 kDa
GenBank Accession NumberBC025300
Gene Symbol FLII
Gene ID (NCBI) 2314
RRIDAB_2920077
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13045
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Flightless I (FliI) is the most evolutionarily conserved member of the gelsolin superfamily of proteins which are key regulators of actin filament assembly and turnover. FliI comprises an N-terminal leucine-rich repeat (LRR) domain which is not present in other gelsolin family members, and the LRR domain may enable interactions between FliI and other molecules involved in signal transduction, thereby spatially integrating signaling and actin remodeling functions. This protein was originally found in Drosophila and participates in the embryonic development, while mammalian FliI protein was involved in the regulation of wound repair,skin barrier development. Studies recently demonstrated that FliI protein associated with colorectal cancer, hepatocellular and prostate cancer (PMID:30091651; 28498392).

实验方案

Product Specific Protocols
IF protocol for CL594 Flightless I antibody CL594-67039Download protocol
Standard Protocols
Click here to view our Standard Protocols