CoraLite®594-conjugated Flightless I Monoclonal antibody

Flightless I Monoclonal Antibody for IF
Cat No. CL594-67039

产品说明书

CloneNo. 2F9C8

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat

应用

IF

FLI, Fli1, FLII, FLIL, Protein flightless 1 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Flightless I Ready-To-Use IHC Kit. Flightless I primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67039-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67039 targets Flightless I in IF applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 IF Application Description
经测试反应性 Human, Mouse, Rat
免疫原 Flightless I fusion protein Ag26865 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 flightless I homolog (Drosophila)
别名 FLI, Fli1, FLII, FLIL, Protein flightless 1 homolog
计算分子量 1269 aa, 145 kDa
观测分子量 145-150 kDa
GenBank蛋白编号BC025300
基因名称 Flightless I
Gene ID (NCBI) 2314
RRIDAB_2920077
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13045
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Flightless I (FliI) is the most evolutionarily conserved member of the gelsolin superfamily of proteins which are key regulators of actin filament assembly and turnover. FliI comprises an N-terminal leucine-rich repeat (LRR) domain which is not present in other gelsolin family members, and the LRR domain may enable interactions between FliI and other molecules involved in signal transduction, thereby spatially integrating signaling and actin remodeling functions. This protein was originally found in Drosophila and participates in the embryonic development, while mammalian FliI protein was involved in the regulation of wound repair,skin barrier development. Studies recently demonstrated that FliI protein associated with colorectal cancer, hepatocellular and prostate cancer (PMID:30091651; 28498392).

实验方案

Product Specific Protocols
IF protocol for CL594 Flightless I antibody CL594-67039Download protocol
Standard Protocols
Click here to view our Standard Protocols
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