验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, A549 cells, NCI-H1299 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells, HepG2 cells, MCF-7 cells, NCCIT cells, HT-1080 cells, LNCaP cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
67039-1-Ig targets Flightless I in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, Mouse, Rat |
| 文献引用反应性 | human |
| 免疫原 | Flightless I fusion protein Ag26865 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | flightless I homolog (Drosophila) |
| 别名 | FLI, Fli1, Flightless I, FLII, FLIL, Protein flightless 1 homolog |
| 计算分子量 | 1269 aa, 145 kDa |
| 观测分子量 | 145-150 kDa |
| GenBank蛋白编号 | BC025300 |
| 基因名称 | Flightless I |
| Gene ID (NCBI) | 2314 |
| RRID | AB_2882353 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13045 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Flightless I (FliI) is the most evolutionarily conserved member of the gelsolin superfamily of proteins which are key regulators of actin filament assembly and turnover. FliI comprises an N-terminal leucine-rich repeat (LRR) domain which is not present in other gelsolin family members, and the LRR domain may enable interactions between FliI and other molecules involved in signal transduction, thereby spatially integrating signaling and actin remodeling functions. This protein was originally found in Drosophila and participates in the embryonic development, while mammalian FliI protein was involved in the regulation of wound repair,skin barrier development. Studies recently demonstrated that FliI protein associated with colorectal cancer, hepatocellular and prostate cancer (PMID:30091651; 28498392).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Flightless I antibody 67039-1-Ig | Download protocol |
| IHC protocol for Flightless I antibody 67039-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genes Dev Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein | ||
Am J Cancer Res SH3BGRL2 exerts a dual function in breast cancer growth and metastasis and is regulated by TGF-β1. |
