验证数据展示
经过测试的应用
| Positive WB detected in | mouse ovary tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60030-1-Ig targets FKBPL in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | FKBPL fusion protein Ag0112 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | FK506 binding protein like |
| 别名 | DIR1, FK506 binding protein like, FKBPL, NG7, WISP39 |
| 计算分子量 | 349 aa, 38 kDa |
| 观测分子量 | 42-48 kDa |
| GenBank蛋白编号 | BC004168 |
| 基因名称 | FKBPL |
| Gene ID (NCBI) | 63943 |
| RRID | AB_2103258 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9UIM3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This protein is thought to have a potential role in the induced radioresistance, probably by increasing the rate of DNA repair in cells exposed to X rays. Also it appears to have some involvement in general stress response in the control of the cell cycle. It regulates p21 protein stability by binding to Hsp90 and p21. FKBPL is an estrogen-inducible gene that acts as a cochaperone in ERα/Hsp90 molecular complexes; furthermore, FKBPL levels may be both a prognostic indicator and determinant of response to endocrine therapy. (PMID:20103631, 15664193)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FKBPL antibody 60030-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |