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验证数据展示

  • WB analysis of HeLa using 23369-1-AP

    WB analysis of HeLa using 23369-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23369-1-AP (FKBPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 23369-1-AP

    IF Staining of HeLa using 23369-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FKBPL antibody (23369-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human ovary tumor using 23369-1-AP

    IHC staining of human ovary tumor using 23369-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23369-1-AP (FKBPL Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 23369-1-AP

    IHC staining of human ovary tumor using 23369-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23369-1-AP (FKBPL Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 23369-1-AP

    FC experiment of HeLa using 23369-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug FKBPL Polyclonal antibody (23369-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

FKBPL Polyclonal antibody

FKBPL Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 23369-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

WISp39, WAF-1/CIP1 stabilizing protein 39, NG7, FK506-binding protein-like, DIR1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23369-1-AP targets FKBPL in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 FKBPL fusion protein Ag19702 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FK506 binding protein like
别名 WISp39, WAF-1/CIP1 stabilizing protein 39, NG7, FK506-binding protein-like, DIR1
计算分子量 349 aa, 38 kDa
观测分子量 42-48 kDa
GenBank蛋白编号BC004168
基因名称 FKBPL
Gene ID (NCBI) 63943
RRIDAB_2879267
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UIM3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193) .It can be detected the band between 38 kDa and 48 kDa by western blot.

实验方案

Product Specific Protocols
WB protocol for FKBPL antibody 23369-1-APDownload protocol
IHC protocol for FKBPL antibody 23369-1-APDownload protocol
IF protocol for FKBPL antibody 23369-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols