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FKBPL Polyclonal antibody

FKBPL Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 23369-1-AP

Print datasheet

Synonyms

DIR1, FK506 binding protein like, FKBPL, NG7, WISP39

验证数据展示

  • WB analysis of HeLa using 23369-1-AP

    WB analysis of HeLa using 23369-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23369-1-AP (FKBPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 23369-1-AP

    IHC staining of human ovary tumor using 23369-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23369-1-AP (FKBPL Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 23369-1-AP

    IHC staining of human ovary tumor using 23369-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23369-1-AP (FKBPL Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 23369-1-AP

    IF Staining of HeLa using 23369-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23369-1-AP (FKBPL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23369-1-AP is FKBPL Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human
Immunogen FKBPL fusion protein Ag19702 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FK506 binding protein like
Synonyms DIR1, FK506 binding protein like, FKBPL, NG7, WISP39
Calculated Molecular Weight 349 aa, 38 kDa
Observed Molecular Weight 42-48 kDa
GenBank Accession NumberBC004168
Gene Symbol FKBPL
Gene ID (NCBI) 63943
RRIDAB_2879267
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UIM3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193) .It can be detected the band between 38 kDa and 48 kDa by western blot.

实验方案

Product Specific Protocols
WB protocol for FKBPL antibody 23369-1-APDownload protocol
IHC protocol for FKBPL antibody 23369-1-APDownload protocol
IF protocol for FKBPL antibody 23369-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols