• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 86189-2-RR (same clone as 86189-2-PBS)

    WB analysis using 86189-2-RR (same clone as 86189-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86189-2-RR (FKBP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86189-2-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 86189-2-RR (same clone as 86189-2-PBS)

    IF Staining of U2OS using 86189-2-RR (same clone as 86189-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FKBP5 antibody (86189-2-RR, Clone: 250798G8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86189-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02289-1

    Cytometric bead array sample test of MP02289-1

    The mean FKBP5 concentration was determined to be 473.3 ng/mL in K-562 cell extract based on a 3.00 mg/mL extract load.

  • Cytometric bead array standard curve of MP02289-1

    Cytometric bead array standard curve of MP02289-1

    Cytometric bead array standard curve of MP02289-1, FKBP5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86189-3-PBS. Detection antibody: 86189-2-PBS. Standard: Ag5337. Range: 1.563-200 ng/mL.

  • Affinity and Kinetic Characterization of 86189-2-RR

    Affinity and Kinetic Characterization of 86189-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86189-2-RR against Human FKBP5 were performed. The affinity constant is 0.957 nM.

FKBP5 Recombinant monoclonal antibody, PBS Only (Detector)

FKBP5 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86189-2-PBS

产品说明书

CloneNo. 250798G8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

FKBP-51, 51 kDa FK506-binding protein, 54 kDa progesterone receptor-associated immunophilin, AIG6, Androgen-regulated protein 6

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy FKBP5 Ready-To-Use IHC Kit. FKBP5 primary antibody included.

产品信息

86189-2-PBS targets FKBP5 as part of a matched antibody pair:

MP02289-1: 86189-3-PBS capture and 86189-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5337

Product name: Recombinant human FKBP5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-375 aa of BC042605

Sequence: MTTDEGAKNNEESPTATVAEQGEDITSKKDRGVLKIVKRVGNGEETPMIGDKVYVHYKGKLSNGKKFDSSHDRNEPFVFSLGKGQVIKAWDIGVATMKKGEICHLLCKPEYAYGSAGSLPKIPSNATLFFEIELLDFKGEDLFEDGGIIRRTKRKGEGYSNPNEGATVEIHLEGRCGGRMFDCRDVAFTVGEGEDHDIPIGIDKALEKMQREEQCILYLGPRYGFGEAGKPKFGIEPNAELIYEVTLKSFEKAKESWEMDTKEKLEQAAIVKEKGTVYFKGGKYMQAVIQYGKIVSWLEMEYGLSEKESKASESFLLAAFLNLAMCYLKLREYTKAVECCDKALGLDSANEKGLYRRGEAQLLMNEFESAKGDFE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 FK506 binding protein 5
别名 FKBP-51, 51 kDa FK506-binding protein, 54 kDa progesterone receptor-associated immunophilin, AIG6, Androgen-regulated protein 6
计算分子量 457 aa, 51 kDa
观测分子量51 kDa
GenBank蛋白编号BC042605
基因名称 FKBP5
Gene ID (NCBI) 2289
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13451
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FKBP5, also known as FKBP51, belongs to the family of immunophilins, FK506 binding proteins (FKBPs). FKBP5 is a negative regulator of GR activity and plays a key role in the termination of the stress response by GRs. FKBP5 limits GR function by decreasing ligand-binding sensitivity, thereby delaying nuclear translocation and reducing GR-dependent transcriptional activity. The known functions of FKBP5 include the regulation of steroid hormone receptors, inhibiting apoptosis in cancer cells, promoting Akt1 dephosphorylation, and inhibiting the Akt1 pathways, to name a few. FKBP5 is widely expressed and the calculated molecular weight is 51 kDa (PMID: 21119664, PMID: 34077736, PMID: 37154033).

||
New chat Able
正在加载，请稍候...