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FKBP2 Polyclonal antibody

FKBP2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11700-1-AP

Print datasheet

Synonyms

13 kDa FK506 binding protein, 13 kDa FKBP, FK506 binding protein 2, FK506 binding protein 2, 13kDa, FKBP 13, FKBP 2, FKBP13, FKBP2, Immunophilin FKBP13, PPIase, PPIase FKBP2, Rotamase

验证数据展示

  • WB analysis of mouse cerebellum using 11700-1-AP

    WB analysis of mouse cerebellum using 11700-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 11700-1-AP (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 11700-1-AP

    IHC staining of human prostate cancer using 11700-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11700-1-AP (FKBP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 11700-1-AP

    IHC staining of human prostate cancer using 11700-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11700-1-AP (FKBP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 11700-1-AP

    WB analysis of HeLa using 11700-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11700-1-AP (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse cerebellum tissue, HeLa cells, MCF-7 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

11700-1-AP targets FKBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen FKBP2 fusion protein Ag2255 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FK506 binding protein 2, 13kDa
Synonyms 13 kDa FK506 binding protein, 13 kDa FKBP, FK506 binding protein 2, FK506 binding protein 2, 13kDa, FKBP 13, FKBP 2, FKBP13, FKBP2, Immunophilin FKBP13, PPIase, PPIase FKBP2, Rotamase
Calculated Molecular Weight 142 aa, 16 kDa
Observed Molecular Weight 13-16 kDa
GenBank Accession NumberBC003384
Gene Symbol FKBP2
Gene ID (NCBI) 2286
RRIDAB_2102876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26885
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FKBP2 is also named as FKBP13 and belongs to the FKBP-type PPIase family. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. FKBP2 has a 21-amino acid signal peptide and appears to be membrane-associated(PMID:1713687). It is localized to the lumen of the endoplasmic reticulum (ER). FKBP12 and FKBP13 are highly similar proteins, of molecular masses 12 kDa and 13 kDa respectively, with approx.43 % amino acid identity. The strong homology between FKBP12 and FKBP13 suggests that they may share similar biological functions, although, apart from rotamase activity, details relating to the function of either protein are scant(PMID:8373365).

实验方案

Product Specific Protocols
WB protocol for FKBP2 antibody 11700-1-APDownload protocol
IHC protocol for FKBP2 antibody 11700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Physiol

Multiple roles for peptidylglycine α-amidating monooxygenase in the response to hypoxia.

Authors - Vishwanatha K S Rao
View Article
mouseWB

Endocrinology

Changes in Corticotrope Gene Expression Upon Increased Expression of Peptidylglycine α-Amidating Monooxygenase.

Authors - Richard E Mains
View Article