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验证数据展示

  • IF Staining of HepG2 using CL488-10956

    IF Staining of HepG2 using CL488-10956

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 FIS1 antibody (CL488-10956) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated FIS1 Polyclonal antibody

FIS1 Polyclonal Antibody for IF/ICC
Cat No. CL488-10956

产品说明书

宿主/亚型

Rabbit / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC

别名

CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 10956-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10956 targets FIS1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag1409

Product name: Recombinant human FIS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-152 aa of BC009428

Sequence: MEAVLNELVSVEDLLKFEKKFQSEKAAGSVSKSTQFEYAWCLVRSKYNDDIRKGIVLLEELLPKGSKEEQRDYVFYLAVGNYRLKEYEKALKYVRGLLQTEPQNNQAKELERLIDKAMKKDGLVGMAIVGGMALGVAGLAGLIGLAVSKSKS

种属同源性预测
宿主/亚型 Rabbit / IgG1
抗体类别 Polyclonal
产品类型 Antibody
全称 fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
别名 CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11
计算分子量 17 kDa
观测分子量 17 kDa
GenBank蛋白编号BC009428
基因名称 FIS1
Gene ID (NCBI) 51024
RRIDAB_2923730
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3D6
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. This antibody was raised against the full-length of human Fis1 protein, and recognizes endogenous Fis1 protein in various lysates. (PMID: 21257639, 21459773, 23333625)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FIS1 antibody CL488-10956Download protocol
Standard Protocols
Click here to view our Standard Protocols