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验证数据展示

  • IF Staining of HeLa using CL488-86060

    IF Staining of HeLa using CL488-86060

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 FHL2 antibody (CL488-86060, Clone: 250585E1 ) at dilution of 1:200, CL594-phalloidin (red).

  • IF Staining of HSkMC using CL488-86060

    IF Staining of HSkMC using CL488-86060

    Immunofluorescent analysis of (4% PFA) fixed HSkMC cells using CoraLite® Plus 488 FHL2 antibody (CL488-86060, Clone: 250585E1 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated FHL2 Recombinant monoclonal antibody

FHL2 Uni-rAb® Recombinant Antibody for IF/ICC
Cat No. CL488-86060

产品说明书

CloneNo. 250585E1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

AAG11, DRAL, FHL 2, FHL-2, LIM domain protein DRAL

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells, HSkMC cells
Planning an IHC experiment? We recommend our IHCeasy FHL2 Ready-To-Use IHC Kit. FHL2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 86060-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-86060 targets FHL2 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag29655

Product name: Recombinant human FHL2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-279 aa of BC014397

Sequence: MTERFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGKDI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 four and a half LIM domains 2
别名 AAG11, DRAL, FHL 2, FHL-2, LIM domain protein DRAL
计算分子量 32 kDa
观测分子量30-32 kDa
GenBank蛋白编号BC014397
基因名称 FHL2
Gene ID (NCBI) 2274
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14192
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FHL2(Four and a half LIM domains protein 2), also known as DRAL. It is located in cytoplasm and nucleus. The protein is expressed in skeletal muscle and heart. May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes (PMID: 28717008). The molecular weight of FHL2 is 32 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FHL2 antibody CL488-86060Download protocol
Standard Protocols
Click here to view our Standard Protocols
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