验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HEK-293 cells, A431 cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human stomach tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82508-1-RR targets FH in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag1934 Product name: Recombinant human FH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 204-510 aa of BC003108 Sequence: HEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | fumarate hydratase |
| 别名 | 3K16, EC:4.2.1.2, Fumarase, HsFH |
| 计算分子量 | 510 aa, 55 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC003108 |
| 基因名称 | FH |
| Gene ID (NCBI) | 2271 |
| RRID | AB_3086485 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P07954 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FH(fumarate hydratase) belongs to the class-II fumarase/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts as a tumor suppressor function of FH in sporadic kidney cancer(PMID:21695080). Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FH antibody 82508-1-RR | Download protocol |
| IHC protocol for FH antibody 82508-1-RR | Download protocol |
| IP protocol for FH antibody 82508-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |