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验证数据展示

  • IHC staining of human stomach using 82508-1-RR (same clone as 82508-1-PBS)

    IHC staining of human stomach using 82508-1-RR (same clone as 82508-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82508-1-RR (FH antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FH antibody included
  • IHC staining of human stomach cancer using 82508-1-RR (same clone as 82508-1-PBS)

    IHC staining of human stomach cancer using 82508-1-RR (same clone as 82508-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82508-1-RR (FH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FH antibody included
  • WB analysis using 82508-1-RR (same clone as 82508-1-PBS)

    WB analysis using 82508-1-RR (same clone as 82508-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82508-1-RR (FH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 82508-1-RR (same clone as 82508-1-PBS)

    WB analysis of HepG2 using 82508-1-RR (same clone as 82508-1-PBS)

    WB result of FH antibody (82508-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FH transfected HepG2 cells. This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 82508-1-RR (same clone as 82508-1-PBS)

    IP experiment of mouse liver using 82508-1-RR (same clone as 82508-1-PBS)

    IP result of anti-FH (IP:82508-1-RR, 4ug; Detection:82508-1-RR 1:4000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82508-1-RR

    Affinity and Kinetic Characterization of 82508-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82508-1-RR against Human FH were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

FH Recombinant monoclonal antibody, PBS Only

FH Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 82508-1-PBS

产品说明书

CloneNo. 3K16

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

3K16, EC:4.2.1.2, Fumarase, HsFH

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FH Ready-To-Use IHC Kit. FH primary antibody included.

产品信息

82508-1-PBS targets FH in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1934

Product name: Recombinant human FH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 204-510 aa of BC003108

Sequence: HEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fumarate hydratase
别名 3K16, EC:4.2.1.2, Fumarase, HsFH
计算分子量 510 aa, 55 kDa
观测分子量45 kDa
GenBank蛋白编号BC003108
基因名称 FH
Gene ID (NCBI) 2271
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP07954
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FH(fumarate hydratase) belongs to the class-II fumarase/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts as a tumor suppressor function of FH in sporadic kidney cancer(PMID:21695080). Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

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