验证数据展示
经过测试的应用
| Positive IF/ICC detected in | Raji cells |
For other applications, we recommend the unconjugated version of this antibody, 83352-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83352 targets FGR in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag34944 Product name: Recombinant human FGR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-90 aa of NM_005248 Sequence: MGCVFCKKLEPVATAKEDAGLEGDFRSYGAADHYGPDPTKARPASSFAHIPNYSNFSSQAINPGFLDSGTIRGVSGIGVTLFIALYDYEA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog |
| 别名 | c fgr, c src2, p55 Fgr, p55c fgr, p58c fgr |
| 计算分子量 | 59 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | NM_005248 |
| 基因名称 | FGR |
| Gene ID (NCBI) | 2268 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P09769 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FGR (Tyrosine-protein kinase Fgr) is a member of the Src family of protein tyrosine kinases (PTKs). It's contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 FGR antibody CL488-83352 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
