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验证数据展示

  • IF Staining of HepG2 using CL594-67391

    IF Staining of HepG2 using CL594-67391

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 FGL1 antibody (CL594-67391, Clone: 2B11C6 ) at dilution of 1:200.

CoraLite®594-conjugated FGL1 Monoclonal antibody

FGL1 Monoclonal Antibody for IF/ICC
Cat No. CL594-67391

产品说明书

CloneNo. 2B11C6

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig

应用

IF/ICC

别名

FGL1, fibrinogen like 1, Fibrinogen like protein 1, Hepassocin, HFREP 1, HFREP1, HP 041, LFIRE 1, LFIRE1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67391-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67391 targets FGL1 in IF/ICC applications and shows reactivity with Human, pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, pig
免疫原

CatNo: Ag8581

Product name: Recombinant human FGL1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-312 aa of BC007047

Sequence: MAKVFSFILVTTALTMGREISALEDCAQEQMRLRAQVRLLETRVKQQQVKIKQLLQENEVQFLDKGDENTVIDLGSKRQYADCSEIFNDGYKLSGFYKIKPLQSPAEFSVYCDMSDGGGWTVIQRRSDGSENFNRGWKDYENGFGNFVQKHGEYWLGNKNLHFLTTQEDYTLKIDLADFEKNSRYAQYKNFKVGDEKNFYELNIGEYSGTAGDSLAGNFHPEVQWWASHQRMKFSTWDRDHDNYEGNCAEEDQSGWWFNRCHSANLNGVYYSGPYTAKTDNGIVWYTWHGWWYSLKSVVMKIRPNDFIPNVI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 fibrinogen-like 1
别名 FGL1, fibrinogen like 1, Fibrinogen like protein 1, Hepassocin, HFREP 1, HFREP1, HP 041, LFIRE 1, LFIRE1
计算分子量 312 aa, 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC007047
基因名称 FGL1
Gene ID (NCBI) 2267
RRIDAB_3673517
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ08830
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibrinogen-like 1 (FGL1), also termed hepatocyte-derived fibrinogen-related protein 1 (HFREP1) or hepassocin, is a predominantly liver expressed protein that has been implicated as both a hepatoprotectant and a hepatocyte mitogen. FGL1 expression is decreased in hepatocellular carcinoma (HCC), and it may play a role in the development of hepatocellular carcinomas.

实验方案

Product Specific Protocols
IF protocol for CL594 FGL1 antibody CL594-67391Download protocol
Standard Protocols
Click here to view our Standard Protocols