验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, human liver tissue, HepG2 cells, mouse thymus tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human hepatocirrhosis tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16000-1-AP targets FGL1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag8581 Product name: Recombinant human FGL1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-312 aa of BC007047 Sequence: MAKVFSFILVTTALTMGREISALEDCAQEQMRLRAQVRLLETRVKQQQVKIKQLLQENEVQFLDKGDENTVIDLGSKRQYADCSEIFNDGYKLSGFYKIKPLQSPAEFSVYCDMSDGGGWTVIQRRSDGSENFNRGWKDYENGFGNFVQKHGEYWLGNKNLHFLTTQEDYTLKIDLADFEKNSRYAQYKNFKVGDEKNFYELNIGEYSGTAGDSLAGNFHPEVQWWASHQRMKFSTWDRDHDNYEGNCAEEDQSGWWFNRCHSANLNGVYYSGPYTAKTDNGIVWYTWHGWWYSLKSVVMKIRPNDFIPNVI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fibrinogen-like 1 |
| 别名 | FGL1, fibrinogen like 1, Fibrinogen like protein 1, Hepassocin, HFREP 1, HFREP1, HP 041, HPS, LFIRE 1, LFIRE1 |
| 计算分子量 | 312 aa, 36 kDa |
| 观测分子量 | 34 kDa, 68 kDa |
| GenBank蛋白编号 | BC007047 |
| 基因名称 | FGL1 |
| Gene ID (NCBI) | 2267 |
| RRID | AB_2103936 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q08830 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FGL1 is a 68 kDa protein that comprises two disulfide linked 34 kDa homodimers. FGL1 is a predominantly liver expressed protein that has been implicated as both a hepatoprotectant and a hepatocyte mitogen. FGL1 expression is decreased in hepatocellular carcinoma (HCC), and it may play a role in the development of hepatocellular carcinomas.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for FGL1 antibody 16000-1-AP | Download protocol |
| IP protocol for FGL1 antibody 16000-1-AP | Download protocol |
| WB protocol for FGL1 antibody 16000-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Immunol Fibrinogen-like protein 1 promotes liver-resident memory T-cell exhaustion in hepatocellular carcinoma | ||
J Nanobiotechnology Regulating the immunosuppressive tumor microenvironment to enhance breast cancer immunotherapy using pH-responsive hybrid membrane-coated nanoparticles. | ||
Int J Mol Sci Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells. | ||
J Clin Endocrinol Metab The Hepatic Protection Effects of Hepassocin in Hyperglycemic Crisis. |
