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验证数据展示

  • WB analysis using 17400-1-AP (same clone as 17400-1-PBS)

    WB analysis using 17400-1-AP (same clone as 17400-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17400-1-AP (FGF1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 17400-1-AP (same clone as 17400-1-PBS)

    IHC staining of human colon cancer using 17400-1-AP (same clone as 17400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FGF1 antibody included
  • IHC staining of human colon cancer using 17400-1-AP (same clone as 17400-1-PBS)

    IHC staining of human colon cancer using 17400-1-AP (same clone as 17400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FGF1 antibody included
  • IF Staining of HepG2 using 17400-1-AP (same clone as 17400-1-PBS)

    IF Staining of HepG2 using 17400-1-AP (same clone as 17400-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FGF1 antibody (17400-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

FGF1 Polyclonal antibody, PBS Only

FGF1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17400-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

FGF-1, Endothelial cell growth factor, Fibroblast growth factor 1, HBGF-1, Heparin-binding growth factor 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FGF1 Ready-To-Use IHC Kit. FGF1 primary antibody included.

产品信息

17400-1-PBS targets FGF1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11366

Product name: Recombinant human FGF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-155 aa of BC032697

Sequence: MAEGEITTFTALTEKFNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fibroblast growth factor 1 (acidic)
别名 FGF-1, Endothelial cell growth factor, Fibroblast growth factor 1, HBGF-1, Heparin-binding growth factor 1
计算分子量 155 aa, 17 kDa
观测分子量 17 kDa
GenBank蛋白编号BC032697
基因名称 FGF1
Gene ID (NCBI) 2246
RRIDAB_2103758
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05230
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fibroblast growth factor 1 (FGF1), also known as acidic fibroblast growth factor (aFGF) and heparin-binding growth factor 1 (HBGF-1), is a 17-18 kDa signaling protein. As a member of the FGF family, it possesses broad mitogenic and cell survival activities, playing critical roles in embryonic development, tissue repair, angiogenesis, and organogenesis. FGF1 exerts its pleiotropic effects by binding to and activating its tyrosine kinase receptors, FGFR1 through FGFR4, in a process that is potentiated by cell-surface heparan sulfate proteoglycans. This activation initiates several downstream signaling cascades, including the MAPK/ERK and AKT pathways. Originally purified in 1975 and distinguished from its homolog FGF2 by its acidic isoelectric point, FGF1 is unique in that it lacks a classical signal peptide and is secreted via a non-classical, stress-induced pathway involving S100A13 and SYT1. Beyond its well-established extracellular functions in wound healing and as a potent inducer of DNA synthesis, FGF1 also possesses intracellular activities and can translocate to the nucleus to influence cell survival.

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