验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse spinal cord tissue, mouse heart tissue, rat heart tissue, rat brain tissue |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, human lung cancer tissue, mouse brain tissue, rat brain tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy FGF1 Ready-To-Use IHC Kit. FGF1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17400-1-AP targets FGF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 |
CatNo: Ag11366 Product name: Recombinant human FGF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-155 aa of BC032697 Sequence: MAEGEITTFTALTEKFNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fibroblast growth factor 1 (acidic) |
| 别名 | FGF-1, Endothelial cell growth factor, Fibroblast growth factor 1, HBGF-1, Heparin-binding growth factor 1 |
| 计算分子量 | 155 aa, 17 kDa |
| 观测分子量 | 17 kDa |
| GenBank蛋白编号 | BC032697 |
| 基因名称 | FGF1 |
| Gene ID (NCBI) | 2246 |
| RRID | AB_2103758 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P05230 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for FGF1 antibody 17400-1-AP | Download protocol |
| IHC protocol for FGF1 antibody 17400-1-AP | Download protocol |
| WB protocol for FGF1 antibody 17400-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuropharmacology Non-mitogenic fibroblast growth factor 1 protects against ischemic stroke by regulating microglia/macrophage polarization through Nrf2 and NF-κB pathways. | ||
Life Sci A derivant of ginsenoside CK and its inhibitory effect on hepatocellular carcinoma. | ||
Physiol Rep Sitagliptin therapy improves myocardial perfusion and arteriolar collateralization in chronically ischemic myocardium: A pilot study | ||
J Cereb Blood Flow Metab Saturated fatty acids stimulate cytokine production in tanycytes via the PP2Ac-dependent signaling pathway | ||
Adv Sci (Weinh) Hepatocyte-Derived FGF1 Alleviates Isoniazid and Rifampicin-Induced Liver Injury by Regulating HNF4α-Mediated Bile Acids Synthesis
| ||
Acta Pharmacol Sin Madecassoside mitigates acute myocardial infarction injury by activating the PKCB/SPARC signaling pathway |
