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FGD1 Polyclonal antibody

FGD1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13141-1-AP

Print datasheet

Synonyms

AAS, FGD1, FGDY, Rho/Rac GEF, ZFYVE3

验证数据展示

  • WB analysis using 13141-1-AP

    WB analysis using 13141-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13141-1-AP (FGD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 13141-1-AP

    WB analysis using 13141-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13141-1-AP (FGD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 13141-1-AP

    IHC staining of mouse kidney using 13141-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13141-1-AP (FGD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inBxPC-3 cells, SK-N-SH cells, U2OS cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13141-1-AP targets FGD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen FGD1 fusion protein Ag3861 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FYVE, RhoGEF and PH domain containing 1
Synonyms AAS, FGD1, FGDY, Rho/Rac GEF, ZFYVE3
Calculated Molecular Weight 901 aa, 91 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC034530
Gene Symbol FGD1
Gene ID (NCBI) 2245
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP98174
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FGD1 is also named as FGDY (Faciogenital dysplasia 1 protein), ZFYVE3 (Zinc finger FYVE domain-containing protein 3), Rho/Rac guanine nucleotide exchange factor FGD1 (Rho/Rac GEF), FYVE, RhoGEF and PH domain-containing protein 1. FGD1 activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. And FGD1 plays a role in regulating the actin cytoskeleton and cell shape. (PMID: 8969170). FGD1 was markedly overexpressed and might be a prognostic biomarker in osteosarcoma patient specimens (PMID: 32194840). FGD1 is specific for the Rho GTPase cell division cycle 42 (CDC42) (PMID: 22854039).

实验方案

Product Specific Protocols
WB protocol for FGD1 antibody 13141-1-APDownload protocol
IHC protocol for FGD1 antibody 13141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols