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CoraLite® Plus 488-conjugated FER Monoclonal antibody

FER Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E3H2

Cat No : CL488-67671

Print datasheet

Synonyms

FER, p94 Fer, Proto oncogene c Fer, TYK3, Tyrosine kinase 3, Tyrosine protein kinase Fer

验证数据展示

  • FC experiment of HeLa using CL488-67671

    FC experiment of HeLa using CL488-67671

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FER (CL488-67671, Clone:1E3H2) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy FER Ready-To-Use IHC Kit. FER primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67671-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67671 targets FER in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra)
Tested Reactivity Human
Immunogen FER fusion protein Ag19176 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name fer (fps/fes related) tyrosine kinase
Synonyms FER, p94 Fer, Proto oncogene c Fer, TYK3, Tyrosine kinase 3, Tyrosine protein kinase Fer
Calculated Molecular Weight 822 aa, 95 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC017060
Gene Symbol FER
Gene ID (NCBI) 2241
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16591
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FER(Tyrosine-protein kinase Fer) is also named as TYK3 and belongs to the protein kinase superfamily. Tyrosine-protein kinase acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. This protein has 3 isoforms produced by alternative promoter usage and alternative splicing.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 FER antibody CL488-67671Download protocol
Standard Protocols
Click here to view our Standard Protocols