• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HeLa using CL647-84916-5

    IF Staining of HeLa using CL647-84916-5

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 FEN1 antibody (CL647-84916-5, Clone: 242330C10 ) at dilution of 1:200.

  • IF Staining of NIH/3T3 using CL647-84916-5

    IF Staining of NIH/3T3 using CL647-84916-5

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CoraLite® Plus 647 FEN1 antibody (CL647-84916-5, Clone: 242330C10 ) at dilution of 1:200.

CoraLite® Plus 647-conjugated FEN1 Recombinant antibody

FEN1 Recombinant Antibody for IF/ICC
Cat No. CL647-84916-5

产品说明书

CloneNo. 242330C10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

242330C10, EC:3.1.-.-, FEN 1, FEN-1, Flap structure-specific endonuclease 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHeLa cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy FEN1 Ready-To-Use IHC Kit. FEN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84916-5-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-84916-5 targets FEN1 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 FEN1 fusion protein Ag6552 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 flap structure-specific endonuclease 1
别名 242330C10, EC:3.1.-.-, FEN 1, FEN-1, Flap structure-specific endonuclease 1
计算分子量 43 kDa
GenBank蛋白编号BC000323
基因名称 FEN1
Gene ID (NCBI) 2237
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP39748
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FEN1(Flap endonuclease-1) is the prototypical member of the 5'-nuclease superfamily, whose activities span a range of cellular pathways involved in DNA replication and genome maintenance (PMID: 22118811, 21496641, 20929870). FEN1 is a structure-selective metallonuclease essential for Okazaki fragment maturation through efficient removal of 5' flaps resulting from strand displacement during lagging-strand synthesis (PMID: 8144677, 9081985). FEN1 is overexpressed in multiple cancer types, and has been suggested both as a biomarker relating to prognosis and disease progression and as a potential therapeutic target (PMID: 19010819, 16879693, 19596913, 27526030).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 FEN1 antibody CL647-84916-5Download protocol
Standard Protocols
Click here to view our Standard Protocols