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验证数据展示

  • WB analysis using 66529-1-Ig (same clone as 66529-1-PBS)

    WB analysis using 66529-1-Ig (same clone as 66529-1-PBS)

    Untreated and PNGase F-treated lysates of THP-1 cells and Mo7e cells were subjected to SDS PAGE followed by western blot with 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66529-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66529-1-Ig (same clone as 66529-1-PBS)

    IHC staining of human liver cancer using 66529-1-Ig (same clone as 66529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66529-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FCGR2A/CD32A antibody included
  • IHC staining of human liver cancer using 66529-1-Ig (same clone as 66529-1-PBS)

    IHC staining of human liver cancer using 66529-1-Ig (same clone as 66529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66529-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FCGR2A/CD32A antibody included
  • IF Staining of THP-1 using 66529-1-Ig (same clone as 66529-1-PBS)

    IF Staining of THP-1 using 66529-1-Ig (same clone as 66529-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using FCGR2A / CD32a antibody (66529-1-Ig, Clone: 1D3F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66529-1-PBS in a different storage buffer formulation.

FCGR2A / CD32a Monoclonal antibody, PBS Only

FCGR2A / CD32a Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66529-1-PBS

产品说明书

CloneNo. 1D3F1

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IHC, IF/ICC, ELISA

别名

CD32, CD32A, CDw32, Fc gamma RII a, Fc gamma RIIa, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A1, FcRII a, IGFR2, IgG Fc receptor II a

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FCGR2A/CD32A Ready-To-Use IHC Kit. FCGR2A/CD32A primary antibody included.

产品信息

66529-1-PBS targets FCGR2A / CD32a in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag25878

Product name: Recombinant human FCGR2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 44-215 aa of BC020823

Sequence: LEPPWINVLQEDSVTLTCQGARSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIMLRCHSWKDKPLVKVTFFQNGKSQKFSHLDPTFSIPQANHSHSGDYHCTGNIGYTLFSSKPVTITVQVPSMGSSSPM

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIa, receptor (CD32)
别名 CD32, CD32A, CDw32, Fc gamma RII a, Fc gamma RIIa, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A1, FcRII a, IGFR2, IgG Fc receptor II a
计算分子量 35 kDa
观测分子量 42 kDa
GenBank蛋白编号BC020823
基因名称 CD32a
Gene ID (NCBI) 2212
RRIDAB_2881892
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP12318
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

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