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验证数据展示

  • WB analysis using 66529-1-Ig

    WB analysis using 66529-1-Ig

    Untreated and PNGase F-treated lysates of THP-1 cells and Mo7e cells were subjected to SDS PAGE followed by western blot with 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66529-1-Ig

    IHC staining of human liver cancer using 66529-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FCGR2A/CD32A antibody included
  • IHC staining of human liver cancer using 66529-1-Ig

    IHC staining of human liver cancer using 66529-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FCGR2A/CD32A antibody included
  • IF Staining of THP-1 using 66529-1-Ig

    IF Staining of THP-1 using 66529-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using FCGR2A / CD32a antibody (66529-1-Ig, Clone: 1D3F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

FCGR2A / CD32a Monoclonal antibody

FCGR2A / CD32a Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66529-1-Ig

产品说明书

CloneNo. 1D3F1

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CD32, 1D3F1, CD32A, Fc gamma RII a, Fc gamma RIIa

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inTF-1 cells, Raji cells, U-937 cells, Mo7e cells, K-562 cells, THP-1 cells
Positive IHC detected inhuman liver cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells
Planning an IHC experiment? We recommend our IHCeasy FCGR2A/CD32A Ready-To-Use IHC Kit. FCGR2A/CD32A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66529-1-Ig targets FCGR2A / CD32a in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag25878

Product name: Recombinant human FCGR2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 44-215 aa of BC020823

Sequence: LEPPWINVLQEDSVTLTCQGARSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIMLRCHSWKDKPLVKVTFFQNGKSQKFSHLDPTFSIPQANHSHSGDYHCTGNIGYTLFSSKPVTITVQVPSMGSSSPM

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIa, receptor (CD32)
别名 CD32, 1D3F1, CD32A, Fc gamma RII a, Fc gamma RIIa
计算分子量 35 kDa
观测分子量 42 kDa
GenBank蛋白编号BC020823
基因名称 CD32a
Gene ID (NCBI) 2212
RRIDAB_2881892
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP12318
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

实验方案

Product Specific Protocols
IF protocol for FCGR2A / CD32a antibody 66529-1-IgDownload protocol
IHC protocol for FCGR2A / CD32a antibody 66529-1-IgDownload protocol
WB protocol for FCGR2A / CD32a antibody 66529-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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