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FBXW11 Monoclonal antibody

FBXW11 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F5A10

Cat No : 68090-1-Ig

Print datasheet

Synonyms

BTRC2, BTRCP2, Fbw11, FBW1B, FBXW11, FBXW1B, Homologous to Slimb protein, Hos, KIAA0696

验证数据展示

  • WB analysis using 68090-1-Ig

    WB analysis using 68090-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68090-1-Ig (FBXW11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 68090-1-Ig

    IHC staining of human colon cancer using 68090-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68090-1-Ig (FBXW11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FBXW11 antibody included

经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FBXW11 Ready-To-Use IHC Kit. FBXW11 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68090-1-Ig targets FBXW11 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen FBXW11 fusion protein Ag3761 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name F-box and WD repeat domain containing 11
Synonyms BTRC2, BTRCP2, Fbw11, FBW1B, FBXW11, FBXW1B, Homologous to Slimb protein, Hos, KIAA0696
Calculated Molecular Weight 542 aa, 61 kDa
Observed Molecular Weight58-62 kDa
GenBank Accession NumberBC026213
Gene Symbol FBXW11
Gene ID (NCBI) 23291
RRIDAB_2918827
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UKB1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXW11 (also known as HOS or β-TrCP2) is a member of F-box family proteins and plays critical role in regulating the ubiquitination of phosphorylated substrates. Abnormal expression of several FBXW11 is involved in the modulation of various biological events, such as cell cycle, differentiation, migration, inflammation, and apoptosis, through targeting multiple different substrates. For instance, FBXW11 could bind to the phosphorylated IκB and β-catenin, promoting their degradation via the ubiquitin-proteasome system. In addition, FBXW11 expression is markedly increased in mouse skin tumors and promotes tumor growth by activating the NF-κB signaling (PMID: 33640602). FBXW11 has 3 isoforms with the molecular mass of 58-62 kDa.

实验方案

Product Specific Protocols
WB protocol for FBXW11 antibody 68090-1-IgDownload protocol
IHC protocol for FBXW11 antibody 68090-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols