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FBXO44 Recombinant antibody

FBXO44 Recombinant Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230229G7

Cat No : 82857-2-RR

Print datasheet

Synonyms

DKFZp781J0852, F box only protein 44, F box protein 44, F box protein FBX30, F box/G domain protein 3, FBG3, FBX30, Fbx44, FBX6A, FBXO44, Fbxo6a

验证数据展示

  • WB analysis using 82857-2-RR

    WB analysis using 82857-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82857-2-RR (FBXO44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 82857-2-RR

    IHC staining of mouse brain using 82857-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82857-2-RR (FBXO44 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 82857-2-RR

    IHC staining of mouse brain using 82857-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82857-2-RR (FBXO44 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 82857-2-RR

    IF Staining of A431 using 82857-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FBXO44 antibody (82857-2-RR, Clone: 230229G7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inK-562 cells, HepG2 cells, A549 cells, HeLa cells, HEK-293 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82857-2-RR targets FBXO44 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IF/ICC, IHC, ELISA
Tested Reactivity Human, mouse
Immunogen FBXO44 fusion protein Ag0977 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name F-box protein 44
Synonyms DKFZp781J0852, F box only protein 44, F box protein 44, F box protein FBX30, F box/G domain protein 3, FBG3, FBX30, Fbx44, FBX6A, FBXO44, Fbxo6a
Calculated Molecular Weight 26 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC007832
Gene Symbol FBXO44
Gene ID (NCBI) 93611
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H4M3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXO44 is a member of the F-box protein family that shares a function as substrate recognition factors for the SKP1-CUL1-F-box (SCF)-type ubiquitin ligase. Several human FBXs (FBXO2, FBXO6, FBXO17, FBXO27, and FBXO44) share a conserved G domain (also known as FBA domain), which is responsible for recognizing N-glycan moieties by most FBXs in this group except FBXO44. (PMID: 25970626, PMID: 23086937)

实验方案

Product Specific Protocols
WB protocol for FBXO44 antibody 82857-2-RRDownload protocol
IHC protocol for FBXO44 antibody 82857-2-RRDownload protocol
IF protocol for FBXO44 antibody 82857-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols